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Recombinant Human ARL6IP1 293 Cell Lysate

Cat.No. : ARL6IP1-8707HCL
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  • Gene Information
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Description : Antigen standard for ADP-ribosylation factor-like 6 interacting protein 1 (ARL6IP1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ARL6IP1 ADP-ribosylation factor-like 6 interacting protein 1 [ Homo sapiens ]
Official Symbol : ARL6IP1
Synonyms : ARL6IP1; ADP-ribosylation factor-like 6 interacting protein 1; ADP ribosylation factor like 6 interacting protein , ARL6IP; ADP-ribosylation factor-like protein 6-interacting protein 1; AIP1; ARMER; KIAA0069; aip-1; ARL-6-interacting protein 1; apoptotic regulator in the membrane of the endoplasmic reticulum; ARL6IP;
Gene ID : 23204
mRNA Refseq : NM_015161
Protein Refseq : NP_055976
MIM : 607669
UniProt ID : Q15041
Chromosome Location : 16p12-p11.2
Function : protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Can dysregulation of ARL6IP1 contribute to human diseases? 10/31/2022

Dysregulation of ARL6IP1 can lead to defects in primary cilia formation and function, which have been associated with various human diseases known as ciliopathies. These include Bardet-Biedl syndrome (BBS), Joubert syndrome (JBTS), Meckel syndrome, and others. In these conditions, mutations or loss of function in genes encoding ciliary proteins, including ARL6IP1, can result in developmental abnormalities and organ dysfunction.

Is there any therapeutic potential in targeting ARL6IP1? 04/14/2022

The therapeutic potential of targeting ARL6IP1 is not well established yet. However, since primary cilia dysfunction has been associated with various diseases, understanding the role of ARL6IP1 in ciliary biology may provide insights into potential therapeutic interventions for ciliopathies and related disorders.

How is ARL6IP1 implicated in retinal diseases? 04/06/2022

Dysfunction of primary cilia in the retina has been associated with certain retinal degenerative diseases, including retinitis pigmentosa. Since ARL6IP1 is an important player in primary cilia formation and maintenance, alterations in its expression or function can contribute to these conditions by disrupting the normal signaling and trafficking processes in retinal cells.

Is ARL6IP1 expression tissue-specific? 02/09/2021

The expression of ARL6IP1 is not strictly tissue-specific but shows variations across different cell types and tissues. It is widely expressed in various organs and cell types, with higher expression observed in tissues rich in primary cilia, such as the retina, kidney, and brain. However, it is also expressed in non-ciliated cells, suggesting additional functions beyond ciliary biology.

What role does ARL6IP1 play in primary cilia formation? 12/10/2020

ARL6IP1 is an essential component for the formation and maintenance of primary cilia. It functions as a linker between the centrosome and the ciliary membrane, facilitating the transport of proteins required for ciliary assembly and signaling. Loss or dysfunction of ARL6IP1 can disrupt primary cilia formation, leading to various developmental and cellular abnormalities.

Are there any known interacting partners of ARL6IP1? 03/30/2018

Yes, ARL6IP1 has been shown to interact with several proteins. These include ARL6, BBSome proteins (BBS1, BBS4, BBS5, and BBS8), MKS1 (Meckel syndrome type 1 protein), NPHP3 (nephrocystin-3), and PCM1 (pericentriolar material 1), among others. These interactions are important for its proper function in primary cilia biology.

Are there any animal models available to study ARL6IP1 function? 07/24/2017

Yes, several animal models have been used to investigate the function of ARL6IP1. These include knockout mice lacking the Arl6ip1 gene, zebrafish models with Arl6ip1 depletion, and other genetically modified organisms. These models help researchers understand the role of ARL6IP1 in embryonic development, cilia formation, and associated diseases.

Can ARL6IP1 interact with other proteins? 05/25/2017

Yes, ARL6IP1 can interact with several proteins involved in cilia formation and function. It has been shown to interact with the small GTPase ARL6, BBSome proteins, and other ciliary trafficking factors. These interactions are important for the proper localization and function of ARL6IP1.

Does ARL6IP1 have any roles outside of primary cilia biology? 04/30/2017

While ARL6IP1 is primarily known for its roles in primary cilia biology, emerging evidence suggests that it may have additional functions. It has been implicated in Golgi organization and vesicular trafficking pathways unrelated to cilia. Further research is needed to fully understand the extent of ARL6IP1's functional contributions in various cellular processes.

Are there any known genetic disorders associated with ARL6IP1 mutations? 02/07/2016

Currently, there are no known genetic disorders specifically associated with ARL6IP1 mutations. However, alterations in primary cilia function, which can result from dysregulation of ARL6IP1, are associated with ciliopathies such as Bardet-Biedl syndrome (BBS) and Joubert syndrome (JBTS).

Customer Reviews (10)

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Reviews
09/30/2022

    They have a deep understanding of the ARL6IP1 protein and can provide valuable insights into its characteristics, properties, and optimal experimental conditions.

    08/05/2022

      Using ARL6IP1 protein in trials provides advantages such as its involvement in cellular processes and its disease associations.

      09/21/2021

        Manufacturers can enhance the research process by providing high-quality ARL6IP1 protein, technical support, customization options, and potentially collaborative opportunities.

        11/16/2020

          the manufacturer offers outstanding technical support, serving as a valuable resource to resolve any issues that may arise during my research.

          07/31/2020

            This guidance can help researchers design and execute their experiments more effectively, ensuring reliable and accurate results.

            06/14/2020

              The manufacturer's support can play a crucial role in assisting researchers throughout their trials.

              07/21/2019

                Its reliability and applicability make it an invaluable tool for investigating ARL6IP1 protein's expression, function, and structural characteristics.

                12/01/2018

                  The high-quality ARL6IP1 protein available from manufacturers ensures consistent and reproducible results in WB experiments, making it an essential tool for researchers studying ANO2-related pathways and biological processes.

                  06/02/2018

                    Their knowledgeable support team is well-equipped to address specific inquiries, suggest experimental protocols, and provide guidance throughout the entire research process.

                    06/10/2017

                      A manufacturer can serve as a collaborative partner, engaging in discussions, and exchanging knowledge with researchers.

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