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Recombinant Human APOBEC3F 293 Cell Lysate

Cat.No. : APOBEC3F-8785HCL
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  • Gene Information
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Description : Antigen standard for apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F (APOBEC3F), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : APOBEC3F apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F [ Homo sapiens ]
Official Symbol : APOBEC3F
Synonyms : APOBEC3F; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F; DNA dC->dU-editing enzyme APOBEC-3F; ARP8; BK150C2.4.MRNA; KA6; DNA dC->dU-editing enzyme APOBEC-3F; induced upon T-cell activation; apolipoprotein B mRNA editing enzyme cytidine deaminase; apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F; MGC74891;
Gene ID : 200316
mRNA Refseq : NM_001006666
Protein Refseq : NP_001006667
MIM : 608993
UniProt ID : Q8IUX4
Chromosome Location : 22q13.1-q13.2
Pathway : APOBEC3G mediated resistance to HIV-1 infection, organism-specific biosystem; Disease, organism-specific biosystem; HIV Infection, organism-specific biosystem; Host Interactions of HIV factors, organism-specific biosystem; Vif-mediated degradation of APOBEC3G, organism-specific biosystem;
Function : RNA binding; cytidine deaminase activity; hydrolase activity; metal ion binding; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (17)

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Can APOBEC3F be targeted for therapeutic interventions? 11/25/2022

There is ongoing research to explore the potential of targeting APOBEC3F for therapeutic purposes, particularly in the context of HIV-1 infection. By modulating the activity of APOBEC3F, it may be possible to enhance its antiviral effects and inhibit virus replication.

Are there any factors that can regulate the activity of APOBEC3F? 05/04/2022

Yes, APOBEC3F activity can be regulated by various cellular factors. For example, the Vif protein encoded by HIV-1 can bind to APOBEC3F and target it for degradation, thereby counteracting its antiviral activity.

How does APOBEC3F specifically target retroviruses like HIV-1? 01/17/2022

APOBEC3F interacts with the viral replication machinery during reverse transcription, the process by which the virus converts its RNA genome into DNA. It binds to the single-stranded viral DNA, where it can deaminate cytosines and induce hypermutation specifically in the viral genome.

Can APOBEC3F affect normal cellular DNA? 02/27/2021

Yes, APOBEC3F has the potential to deaminate cytosine residues in cellular DNA as well. However, it is believed that cellular mechanisms and other APOBEC proteins, such as APOBEC3G, work together to limit the damage caused by APOBEC3F to the cellular genome.

Does APOBEC3F have any other functions besides its antiviral activity? 01/06/2021

APOBEC3F has been shown to play a role in controlling endogenous retroelements, which are remnants of ancient retroviral infections that have become part of the human genome. It helps to suppress the activity of retroelements and prevent their harmful effects.

In which cells is APOBEC3F expressed? 05/04/2020

APOBEC3F is expressed primarily in immune cells, such as T cells, B cells, and macrophages.

Are there any known viruses that can counteract APOBEC3F without Vif? 03/21/2020

While APOBEC3F can be counteracted by the Vif protein of certain viruses like HIV-1, there are no known viruses that can directly counteract APOBEC3F activity without the presence of Vif. However, viruses may have other strategies to evade or counteract APOBEC3F through different mechanisms.

Can APOBEC3F be used as a diagnostic or prognostic marker for diseases? 12/29/2019

APOBEC3F expression levels have been investigated as potential diagnostic and prognostic markers in certain cancers, such as hepatocellular carcinoma. However, more research is needed to validate its utility in clinical settings.

Can APOBEC3F be artificially upregulated or enhanced to enhance antiviral activity? 12/07/2019

There have been studies exploring ways to artificially upregulate or enhance APOBEC3F expression to enhance its antiviral activity. For example, researchers have investigated the use of small molecule drugs that can induce APOBEC3F expression to enhance its antiviral effects. However, further research is needed to determine the efficacy and safety of such approaches.

How does APOBEC3F induce hypermutation in retroviruses? 07/29/2018

APOBEC3F deaminates cytosine residues in the viral DNA, leading to the conversion of cytosine to uracil. During DNA replication, uracil is recognized as an error and is likely to be replaced by other nucleotides, resulting in the introduction of mutations in the viral genome.

Are there any known drugs that can modulate APOBEC3F activity? 07/02/2018

Currently, there are no specific drugs available that can directly modulate APOBEC3F activity. However, some drugs that can affect the overall activity of the APOBEC family of proteins, including APOBEC3F, have been investigated. These drugs target cellular pathways that regulate APOBEC protein expression or function, but their specific effects on APOBEC3F need further exploration.

How is APOBEC3F believed to have evolved? 11/20/2017

APOBEC3F and other members of the APOBEC3 family are thought to have evolved as part of an ongoing arms race between retroviruses and host defense mechanisms. The viruses evolved strategies to counteract APOBEC3 proteins, leading to the generation of diverse APOBEC3 variants in the host population.

Are there any known genetic variations in the APOBEC3F gene? 08/27/2017

Yes, there are genetic variations, including single nucleotide polymorphisms (SNPs), identified in the APOBEC3F gene. Some of these variations have been associated with altered susceptibility to viral infections and cancer development.

Are there any therapeutic strategies targeting APOBEC3F? 05/03/2017

There are currently no specific therapeutic strategies targeting APOBEC3F directly. However, understanding its function and interaction with viral pathogens could potentially lead to the development of targeted therapies in the future.

Are there any known inhibitors of APOBEC3F? 02/18/2017

Currently, there are no specific inhibitors of APOBEC3F identified. However, research is being conducted to discover small molecules or peptides that can selectively inhibit the activity of APOBEC3F without affecting other important cellular processes.

Are there any diseases associated with APOBEC3F malfunction? 07/23/2016

APOBEC3F dysfunction has been implicated in certain cancers, including liver and lung cancers. Additionally, some studies have suggested that APOBEC3F activity may be involved in immune-related diseases like systemic lupus erythematosus (SLE).

Can APOBEC3F be targeted for gene therapy approaches? 06/16/2016

There is ongoing research exploring the potential use of APOBEC3F as a target for gene therapy approaches. Strategies could involve modifying or enhancing APOBEC3F function to enhance antiviral activity or selectively target cancer cells. However, this area of research is still in its early stages and requires further development.

Customer Reviews (4)

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Reviews
11/04/2022

    This specificity enables precise quantification and characterization of APOBEC3F and its interactions with other molecules or proteins of interest.

    01/12/2022

      The APOBEC3F protein exhibits a high degree of specificity in various assays, ensuring accurate and reliable results.

      04/10/2020

        Its exceptional performance, coupled with its ability to generate clear and precise protein bands, make it an indispensable tool in achieving accurate and reliable results.

        10/23/2016

          APOBEC3F protein is known for its stability and robustness, making it suitable for a wide range of experimental conditions.

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