"APIP" Related Products

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Recombinant Human APIP 293 Cell Lysate

Cat.No.: APIP-8795HCL
Description: Antigen standard for APAF1 interacting protein (APIP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: APIP APAF1 interacting protein [ Homo sapiens ]
Official Symbol: APIP
Synonyms: APIP; APAF1 interacting protein; probable methylthioribulose-1-phosphate dehydratase; APIP2; CGI 29; Mmrp19; MTRu-1-P dehydratase; APAF1-interacting protein; CGI29; CGI-29; MMRP19; dJ179L10.2;
Gene ID: 51074
mRNA Refseq: NM_015957
Protein Refseq: NP_057041
MIM: 612491
UniProt ID: Q96GX9
Chromosome Location: 11p13
Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Metabolism of polyamines, organism-specific biosystem; Methionine salvage pathway, organism-specific biosystem;
Function: identical protein binding; ion binding; lyase activity; metal ion binding; methylthioribulose 1-phosphate dehydratase activity; protein binding;

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