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Recombinant Human ANXA3 293 Cell Lysate

Cat.No.: ANXA3-8832HCL
Description: Antigen standard for annexin A3 (ANXA3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ANXA3 annexin A3 [ Homo sapiens ]
Official Symbol: ANXA3
Synonyms: ANXA3; annexin A3; ANX3; PAP-III; annexin-3; lipocortin III; 35-alpha calcimedin; calcimedin 35-alpha; Annexin III (lipocortin III); placental anticoagulant protein III; inositol 1,2-cyclic phosphate 2-phosphohydrolase; annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha);
Gene ID: 306
mRNA Refseq: NM_005139
Protein Refseq: NP_005130
MIM: 106490
UniProt ID: P12429
Chromosome Location: 4q21.21
Pathway: Prostaglandin Synthesis and Regulation, organism-specific biosystem;
Function: calcium ion binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity;

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