||Recombinant Human Activated Protein C produced inCHOis a disulfide-linked homodimeric, glycosylated, polypeptide chain having a molecular mass of 55kDa. Activated Protein C Human Recombinant is a serine protease with the same amino acid sequence and glycosilation sites as the human plasma-derived Activated Protein C. The APC is purified by proprietary chromatographic techniques.
||Activated Protein C exerts an antithrombotic effect by inhibiting Factors Va and VIIIa. In vitro data indicate that Activated Protein C has indirect profibrinolytic activity through its ability to inhibit plasminogen activator inhibitor-1 (PAI-1) and limiting generation of activated thrombin-activatable-fibrinolysis-inhibitor. Additionally, in vitro data indicate that Activated Protein C may exert an anti-inflammatory effect by inhibiting human tumor necrosis factor production by monocytes, by blocking leukocyte adhesion to selectins, and by limiting the thrombin-induced inflammatory responses within the microvascular endothelium.
||Chinese Hamster Ovary Cells (CHO).
||Sterile Filtered White lyophilized (freeze-dried) powder.
||Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
||Each mg protein contains 7. 6 mg sodium chloride, 1.5 mg citrate and 6 mg sucrose.
||It is recommended to reconstitute the lyophilized Coagulation factor V in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
||Lyophilized Factor V although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution APC should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.