Recombinant Human AMD1 293 Cell Lysate
Cat.No. : | AMD1-8886HCL |
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- Gene Information
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Description : | Antigen standard for adenosylmethionine decarboxylase 1 (AMD1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AMD1 adenosylmethionine decarboxylase 1 [ Homo sapiens ] |
Official Symbol : | AMD1 |
Synonyms : | AMD1; adenosylmethionine decarboxylase 1; S adenosylmethionine decarboxylase 1; S-adenosylmethionine decarboxylase proenzyme; SAMDC; S-adenosylmethionine decarboxylase 1; AMD; ADOMETDC; FLJ26964; DKFZp313L1234; |
Gene ID : | 262 |
mRNA Refseq : | NM_001033059 |
Protein Refseq : | NP_001028231 |
MIM : | 180980 |
UniProt ID : | P17707 |
Chromosome Location : | 6q21 |
Pathway : | Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; |
Function : | adenosylmethionine decarboxylase activity; adenosylmethionine decarboxylase activity; lyase activity; |
Products Types
◆ Recombinant Protein | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAMD1 interacts with other enzymes, such as ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (SAMDC), in the polyamine biosynthetic pathway, forming a regulatory network.
Alterations in AMD1 expression or activity have been observed in various diseases, including cancer, suggesting its potential as a diagnostic marker or therapeutic target for specific conditions.
AMD1 expression is regulated at the transcriptional level by various factors, including growth factors, hormones, and cellular stressors.
AMD1 represents a potential pharmacological target for modulating polyamine metabolism, and its inhibition has been explored as a therapeutic strategy in diseases such as cancer.
AMD1 deficiency or inhibition can lead to altered polyamine levels and impaired cellular processes, including reduced cell proliferation and compromised cell survival.
AMD1 plays a key role in polyamine biosynthesis, regulating the levels of polyamines such as putrescine, spermidine, and spermine, which are involved in cell growth, proliferation, and differentiation.
AMD1 is an enzyme that catalyzes the conversion of S-adenosylmethionine (SAM) to decarboxylated SAM, which is a crucial step in the biosynthesis of polyamines.
The catalytic activity and substrate specificity of AMD1 are determined by its unique protein structure, including active site residues and cofactor binding sites.
AMD1 activity influences downstream signaling pathways, including mTOR signaling, DNA methylation, and gene expression, which are involved in cell growth and proliferation.
The substrates of AMD1 include SAM, the primary methyl donor in cellular methylation reactions, and its decarboxylated product, which serves as a precursor for polyamine synthesis.
Customer Reviews (3)
Write a reviewCompatibility with high-resolution imaging techniques for detailed analysis.
Accurate measurement of protein turnover rates.
Reduced non-specific binding in antibody-based assays.
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