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Recombinant Human AMD1 293 Cell Lysate

Cat.No. : AMD1-8886HCL
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Description : Antigen standard for adenosylmethionine decarboxylase 1 (AMD1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AMD1 adenosylmethionine decarboxylase 1 [ Homo sapiens ]
Official Symbol : AMD1
Synonyms : AMD1; adenosylmethionine decarboxylase 1; S adenosylmethionine decarboxylase 1; S-adenosylmethionine decarboxylase proenzyme; SAMDC; S-adenosylmethionine decarboxylase 1; AMD; ADOMETDC; FLJ26964; DKFZp313L1234;
Gene ID : 262
mRNA Refseq : NM_001033059
Protein Refseq : NP_001028231
MIM : 180980
UniProt ID : P17707
Chromosome Location : 6q21
Pathway : Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem;
Function : adenosylmethionine decarboxylase activity; adenosylmethionine decarboxylase activity; lyase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How does AMD1 interact with other enzymes or regulatory proteins involved in polyamine metabolism? 10/01/2022

AMD1 interacts with other enzymes, such as ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (SAMDC), in the polyamine biosynthetic pathway, forming a regulatory network.

How does AMD1 expression or activity correlate with disease states, such as cancer, and can it serve as a diagnostic or therapeutic target? 04/19/2022

Alterations in AMD1 expression or activity have been observed in various diseases, including cancer, suggesting its potential as a diagnostic marker or therapeutic target for specific conditions.

How is AMD1 expression regulated in different tissues and under various physiological conditions? 12/26/2020

AMD1 expression is regulated at the transcriptional level by various factors, including growth factors, hormones, and cellular stressors.

Can AMD1 be targeted pharmacologically to modulate polyamine metabolism and potentially treat diseases? 12/25/2020

AMD1 represents a potential pharmacological target for modulating polyamine metabolism, and its inhibition has been explored as a therapeutic strategy in diseases such as cancer.

What is the impact of AMD1 deficiency or inhibition on cell proliferation, differentiation, and survival? 07/25/2020

AMD1 deficiency or inhibition can lead to altered polyamine levels and impaired cellular processes, including reduced cell proliferation and compromised cell survival.

How does AMD1 contribute to polyamine biosynthesis and the regulation of intracellular polyamine levels? 04/13/2020

AMD1 plays a key role in polyamine biosynthesis, regulating the levels of polyamines such as putrescine, spermidine, and spermine, which are involved in cell growth, proliferation, and differentiation.

What is the enzymatic activity of AMD1 and its role in cellular metabolism? 02/22/2020

AMD1 is an enzyme that catalyzes the conversion of S-adenosylmethionine (SAM) to decarboxylated SAM, which is a crucial step in the biosynthesis of polyamines.

What are the structural features of AMD1 that contribute to its catalytic activity and substrate specificity? 01/17/2020

The catalytic activity and substrate specificity of AMD1 are determined by its unique protein structure, including active site residues and cofactor binding sites.

What are the downstream signaling pathways or cellular processes influenced by AMD1 activity? 09/09/2018

AMD1 activity influences downstream signaling pathways, including mTOR signaling, DNA methylation, and gene expression, which are involved in cell growth and proliferation.

What are the substrates of AMD1 and their functional implications in cellular processes? 09/17/2017

The substrates of AMD1 include SAM, the primary methyl donor in cellular methylation reactions, and its decarboxylated product, which serves as a precursor for polyamine synthesis.

Customer Reviews (3)

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Reviews
05/06/2019

    Compatibility with high-resolution imaging techniques for detailed analysis.

    12/29/2018

      Accurate measurement of protein turnover rates.

      10/03/2017

        Reduced non-specific binding in antibody-based assays.

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