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Recombinant Human ALOX5 293 Cell Lysate

Cat.No. : ALOX5-8896HCL
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  • Gene Information
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Description : Antigen standard for arachidonate 5-lipoxygenase (ALOX5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ALOX5 arachidonate 5-lipoxygenase [ Homo sapiens ]
Official Symbol : ALOX5
Synonyms : ALOX5; arachidonate 5-lipoxygenase; 5 LOX; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; 5-LO; 5LPG; LOG5; 5-LOX; MGC163204;
Gene ID : 240
mRNA Refseq : NM_000698
Protein Refseq : NP_000689
MIM : 152390
UniProt ID : P09917
Chromosome Location : 10q11.2
Pathway : Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Eicosanoid Synthesis, organism-specific biosystem; IL-5 Signaling Pathway, organism-specific biosystem; Leukotriene synthesis, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem;
Function : arachidonate 5-lipoxygenase activity; arachidonate 5-lipoxygenase activity; iron ion binding; lipoxygenase activity; metal ion binding; oxidoreductase activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (13)

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On which chromosome is the ALOX5 coding gene? 02/15/2023

The ALOX5 coding gene is located on human chromosome 10.

What are ALOX5 inhibitors? What is their mechanism of action? 12/30/2022

Currently known ALOX5 inhibitors include trypanosomamine, Ritopane, MK-886, etc., whose mechanism of action is to interfere with the expression or function of ALOX5 in different ways, thereby playing an anti-inflammatory or anti-tumor role.

What biological processes is ALOX5 involved in? 12/27/2022

ALOX5 is involved in a variety of biological processes, including apoptosis, cell proliferation, inflammatory response, and cancer metastasis.

What factors can regulate ALOX5 gene expression? 10/20/2022

The gene expression can be regulated by a variety of factors such as prostaglandins, cytokines and interferons.

Which foods inhibit ALOX5 activity? 04/08/2022

Some foods rich in polyphenols, such as cucumbers, tomatoes, grapes, green tea, etc., are thought to inhibit ALOX5 activity.

What cell types have high levels of ALOX5 gene expression? 04/07/2022

In general, the ALOX5 gene is highly expressed in eosinophils and monocytes.

What is the function of the ALOX5 promoter region? 01/08/2022

The ALOX5 promoter region is the starting point of gene transcription and is responsible for mediating the transcription regulation of the ALOX5 gene.

How to inhibit the effect of ALOX5 in tumor treatment with drugs? 12/18/2021

Drugs that block the ALOX5 signaling pathway, such as Zileuton, MK886, and A861, have been shown to inhibit tumor cell proliferation and invasion. Currently, there are also many researchers who are exploring ALOX5 in tumors.

What diseases can ALOX5 gene mutation cause? 01/17/2021

ALOX5 gene mutation may cause asthma, allergic rhinitis, eczema and other allergic diseases.

What is 5-HETE? How is it related to ALOX5? 12/02/2020

5-HETE is one of the representative products of ALOX5 catalyzed synthesis, and is an activator of platelets and neutrophils.

What is the specific mechanism by which ALOX5 mediates the occurrence of human cancer? 11/07/2019

There is no definite answer to this question. Current studies believe that ALOX5 can affect the occurrence, development and metastasis of tumors through multiple ways, including regulating the proliferation, apoptosis, invasion and metastasis of tumor cells.

In what areas can ALOX5 knockout mouse models be used for research? 09/03/2019

ALOX5 knockout mouse models can be used to study a variety of biological processes and diseases, such as immune regulation, inflammation, asthma, eczema, autoimmunity, etc.

How to detect the expression of ALOX5 gene? 07/27/2019

At present, the commonly used methods include real-time fluorescent quantitative PCR, Northern blot, Western blot, etc.

Customer Reviews (3)

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Reviews
05/09/2022

    After ALOX5 application, the background of the Western blot was very clean with no nonspecific signal.

    08/10/2020

      ALOX5 significantly improved the experimental accuracy and made the experimental results more reliable.

      05/07/2020

        I appreciate the stability of ALOX5, which can be kept at room temperature for several days without spoilage.

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