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Recombinant Human ALDH7A1 293 Cell Lysate

Cat.No.: ALDH7A1-8915HCL
Description: Antigen standard for aldehyde dehydrogenase 7 family, member A1 (ALDH7A1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ALDH7A1 aldehyde dehydrogenase 7 family, member A1 [ Homo sapiens ]
Official Symbol: ALDH7A1
Synonyms: ALDH7A1; aldehyde dehydrogenase 7 family, member A1; ATQ1; alpha-aminoadipic semialdehyde dehydrogenase; 26g turgor protein homolog; alpha AASA dehydrogenase; alpha aminoadipic semialdehyde dehydrogenase; antiquitin 1; delta1 piperideine 6 carboxylate dehydrogenease; EPD; P6c dehydrogenase; PDE; antiquitin-1; alpha-AASA dehydrogenase; betaine aldehyde dehydrogenase; delta1-piperideine-6-carboxylate dehydrogenase; FLJ11738; FLJ92814;
Gene ID: 501
mRNA Refseq: NM_001182
Protein Refseq: NP_001173
MIM: 107323
UniProt ID: P49419
Chromosome Location: 5q31
Pathway: Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Ascorbate and aldarate metabolism, organism-specific biosystem; Ascorbate and aldarate metabolism, conserved biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; GABA biosynthesis, eukaryotes, putrescine =>
Function: L-aminoadipate-semialdehyde dehydrogenase activity; aldehyde dehydrogenase (NAD) activity; betaine-aldehyde dehydrogenase activity; oxidoreductase activity; protein binding;

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