"ALDH1A2" Related Products

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Recombinant Human ALDH1A2 293 Cell Lysate

Cat.No.: ALDH1A2-8921HCL
Description: Antigen standard for aldehyde dehydrogenase 1 family, member A2 (ALDH1A2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ALDH1A2?aldehyde dehydrogenase 1 family, member A2 [?Homo sapiens(human) ]
Official Symbol: ALDH1A2
Synonyms: ALDH1A2; aldehyde dehydrogenase 1 family, member A2; RALDH2; RALDH2-T; RALDH(II); retinal dehydrogenase 2; RALDH 2; retinaldehyde-specific dehydrogenase type 2; NP_001193826.1; EC 1.2.1.36; NP_003879.2; NP_733797.1; NP_733798.1
Gene ID: 8854
mRNA Refseq: NM_170696
Protein Refseq: NP_733797
MIM: 603687
UniProt ID: O94788
Chromosome Location: 15q21.3
Pathway: Retinol metabolism, organism-specific biosystem; Retinol metabolism, conserved biosystem; Tryptophan metabolism, organism-specific biosystem; retinoate biosynthesis I, conserved biosystem
Function: 3-chloroallyl aldehyde dehydrogenase activity; retinal binding; retinal dehydrogenase activity

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