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Recombinant Human AGTR2 293 Cell Lysate

Cat.No. : AGTR2-8969HCL
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  • Gene Information
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Description : Antigen standard for angiotensin II receptor, type 2 (AGTR2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AGTR2 angiotensin II receptor, type 2 [ Homo sapiens ]
Official Symbol : AGTR2
Synonyms : AGTR2; angiotensin II receptor, type 2; angiotensin receptor 2; type-2 angiotensin II receptor; AT2; MRX88; angiotensin II type-2 receptor; ATGR2;
Gene ID : 186
mRNA Refseq : NM_000686
Protein Refseq : NP_000677
MIM : 300034
UniProt ID : P50052
Chromosome Location : Xq22-q23
Pathway : ACE Inhibitor Pathway, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem;
Function : G-protein coupled receptor activity; angiotensin type II receptor activity; protein binding; receptor activity; receptor antagonist activity; signal transducer activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Are there any epigenetic modifications associated with AGTR2 expression? 05/08/2022

Epigenetic modifications, such as DNA methylation and histone acetylation, have been implicated in regulating AGTR2 expression. Hypermethylation of the AGTR2 gene promoter has been observed in certain pathological conditions, suggesting a potential mechanism for altered AGTR2 expression and associated diseases.

How does AGTR2 impact cardiac remodeling after myocardial infarction? 09/24/2021

AGTR2 activation can exert anti-fibrotic and anti-hypertrophic effects in the heart after myocardial infarction. AGTR2 activation promotes cardiomyocyte survival, limits adverse remodeling, and improves cardiac function, indicating its therapeutic potential in post-MI cardiac remodeling.

How does AGTR2 interact with other signaling pathways? 09/08/2021

AGTR2 can cross-talk with other receptors and signaling pathways, such as the insulin-like growth factor 1 receptor and the Wnt/β-catenin pathway, to regulate cellular processes.

How is AGTR2 regulated at the transcriptional level? 05/20/2021

Several transcription factors, including Sp1, AP-1, and NF-κB, can bind to the promoter region of AGTR2 and modulate its gene expression.

How is AGTR2 involved in cancer biology? 04/02/2020

AGTR2 may have dual roles in cancer progression, acting as a tumor suppressor or a promoter depending on the specific cancer type.

What post-translational modifications are known to regulate AGTR2 activity? 02/19/2020

Phosphorylation, ubiquitination, and glycosylation are among the post-translational modifications that can modulate AGTR2 function and signaling.

Are there any genetic polymorphisms associated with AGTR2? 02/03/2020

Yes, certain genetic variations in AGTR2 have been linked to various diseases, including hypertension, preeclampsia, and neurological disorders.

What are the downstream signaling pathways activated by AGTR2? 09/01/2019

AGTR2 can activate multiple signaling pathways, including phosphatases, kinases, and nitric oxide synthase.

What are the tissue-specific expression patterns of AGTR2? 01/14/2019

AGTR2 is expressed in various tissues, including the cardiovascular system, brain, kidney, and reproductive organs.

What is the role of AGTR2 in the cardiovascular system? 07/07/2018

AGTR2 has been implicated in cardiovascular development, blood pressure regulation, and vascular remodeling.

Customer Reviews (5)

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Reviews
03/12/2022

    It worked seamlessly in my experiments, yielding consistent and reliable results.

    06/25/2021

      The protein I purchased exceeded my expectations. It was highly pure and performed exceptionally well in my protein-protein interaction studies.

      02/14/2020

        I highly recommend this product. It saved me a significant amount of time and effort in my research, delivering excellent performance.

        09/08/2019

          It was easy to use, and the results were reproducible, giving me confidence in my data.

          11/30/2018

            Exceptional protein. Worth every penny.

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