Recombinant Human AGR3 293 Cell Lysate
Cat.No. : | AGR3-8971HCL |
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- Gene Information
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Description : | Antigen standard for anterior gradient homolog 3 (Xenopus laevis) (AGR3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AGR3 anterior gradient 3 homolog (Xenopus laevis) [ Homo sapiens ] |
Official Symbol : | AGR3 |
Synonyms : | AGR3; anterior gradient 3 homolog (Xenopus laevis); anterior gradient protein 3 homolog; BCMP11; breast cancer membrane protein 11; hAG 3; HAG3; PDIA18; protein disulfide isomerase family A; member 18; AG-3; anterior gradient homolog 3; protein disulfide isomerase family A, member 18; AG3; hAG-3; |
Gene ID : | 155465 |
mRNA Refseq : | NM_176813 |
Protein Refseq : | NP_789783 |
MIM : | 609482 |
UniProt ID : | Q8TD06 |
Chromosome Location : | 7p21.1 |
Function : | alpha-dystroglycan binding; |
Products Types
◆ Recombinant Protein | ||
AGR3-533H | Recombinant Human AGR3 Protein, His-tagged | +Inquiry |
Agr3-1561M | Recombinant Mouse Agr3 Protein, Myc/DDK-tagged | +Inquiry |
AGR3-395M | Recombinant Mouse AGR3 Protein, His (Fc)-Avi-tagged | +Inquiry |
AGR3-518H | Recombinant Human AGR3 Protein, MYC/DDK-tagged | +Inquiry |
AGR3-4422C | Recombinant Chicken AGR3 | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionThe interaction partners of AGR3 can be identified using techniques such as co-immunoprecipitation (Co-IP), yeast two-hybrid assays, or proximity-dependent biotin identification (BioID).
AGR3 is thought to promote cancer cell survival, proliferation, and metastasis by modulating cellular signaling pathways and interacting with other proteins involved in cancer progression.
The exact mechanisms are still being elucidated, but AGR3 has been shown to interact with proteins involved in cell adhesion, invasion, and apoptosis, contributing to cancer phenotypes.
AGR3 protein samples should be stored at -80°C to maintain their stability and prevent degradation. It is advisable to aliquot the protein to avoid repeated freeze-thaw cycles.
The purification of AGR3 protein can be achieved through techniques such as affinity chromatography, size-exclusion chromatography, or ion-exchange chromatography.
AGR3 expression is regulated by androgen receptor signaling in prostate cancer cells, and it may play a role in mediating androgen-dependent growth and survival.
Targeting AGR3 is an area of active research, and potential strategies such as small molecule inhibitors or immunotherapies are being explored for therapeutic interventions.
AGR3 expression can be detected and quantified using techniques such as immunohistochemistry, immunoblotting, and molecular assays like quantitative PCR (qPCR) or RNA sequencing.
Limited animal models, such as mouse models, have been utilized to study AGR3 function in specific tissues or diseases, providing insights into its physiological roles.
Similar to AGR2, AGR3 can be secreted into the extracellular space, where it may interact with other proteins and potentially influence cell signaling and behavior.
Customer Reviews (5)
Write a reviewI would appreciate more detailed documentation accompanying the product.
The proteins offered by this company are of exceptional quality and come at an affordable price, making them an excellent choice for my research needs.
The customer service was exceptional, with prompt assistance and valuable technical support.
This product exceeded my expectations in terms of quality.
This protein helo me to continue my experiments without breaking the bank.
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