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Recombinant Human AGR3 293 Cell Lysate

Cat.No. : AGR3-8971HCL
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  • Gene Information
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Description : Antigen standard for anterior gradient homolog 3 (Xenopus laevis) (AGR3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AGR3 anterior gradient 3 homolog (Xenopus laevis) [ Homo sapiens ]
Official Symbol : AGR3
Synonyms : AGR3; anterior gradient 3 homolog (Xenopus laevis); anterior gradient protein 3 homolog; BCMP11; breast cancer membrane protein 11; hAG 3; HAG3; PDIA18; protein disulfide isomerase family A; member 18; AG-3; anterior gradient homolog 3; protein disulfide isomerase family A, member 18; AG3; hAG-3;
Gene ID : 155465
mRNA Refseq : NM_176813
Protein Refseq : NP_789783
MIM : 609482
UniProt ID : Q8TD06
Chromosome Location : 7p21.1
Function : alpha-dystroglycan binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How can the interaction partners of AGR3 be identified in the laboratory? 02/19/2022

The interaction partners of AGR3 can be identified using techniques such as co-immunoprecipitation (Co-IP), yeast two-hybrid assays, or proximity-dependent biotin identification (BioID).

How does AGR3 contribute to cancer development? 06/02/2021

AGR3 is thought to promote cancer cell survival, proliferation, and metastasis by modulating cellular signaling pathways and interacting with other proteins involved in cancer progression.

What are the molecular mechanisms underlying AGR3's role in cancer? 01/31/2021

The exact mechanisms are still being elucidated, but AGR3 has been shown to interact with proteins involved in cell adhesion, invasion, and apoptosis, contributing to cancer phenotypes.

What are the recommended storage conditions for AGR3 protein samples in the laboratory? 01/28/2021

AGR3 protein samples should be stored at -80°C to maintain their stability and prevent degradation. It is advisable to aliquot the protein to avoid repeated freeze-thaw cycles.

What are the common techniques used to purify AGR3 protein in the laboratory? 08/10/2020

The purification of AGR3 protein can be achieved through techniques such as affinity chromatography, size-exclusion chromatography, or ion-exchange chromatography.

What is the relationship between AGR3 and androgen signaling in prostate cancer? 10/05/2019

AGR3 expression is regulated by androgen receptor signaling in prostate cancer cells, and it may play a role in mediating androgen-dependent growth and survival.

Can AGR3 be targeted for therapeutic interventions? 08/12/2019

Targeting AGR3 is an area of active research, and potential strategies such as small molecule inhibitors or immunotherapies are being explored for therapeutic interventions.

How can AGR3 expression be measured in experimental studies? 02/23/2019

AGR3 expression can be detected and quantified using techniques such as immunohistochemistry, immunoblotting, and molecular assays like quantitative PCR (qPCR) or RNA sequencing.

Are there any animal models available to study AGR3 function? 01/14/2019

Limited animal models, such as mouse models, have been utilized to study AGR3 function in specific tissues or diseases, providing insights into its physiological roles.

Does AGR3 have any extracellular functions? 04/12/2018

Similar to AGR2, AGR3 can be secreted into the extracellular space, where it may interact with other proteins and potentially influence cell signaling and behavior.

Customer Reviews (5)

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Reviews
06/20/2022

    I would appreciate more detailed documentation accompanying the product.

    04/23/2021

      The proteins offered by this company are of exceptional quality and come at an affordable price, making them an excellent choice for my research needs.

      07/22/2020

        The customer service was exceptional, with prompt assistance and valuable technical support.

        10/22/2019

          This product exceeded my expectations in terms of quality.

          05/04/2019

            This protein helo me to continue my experiments without breaking the bank.

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