Recombinant Human AGFG2 293 Cell Lysate


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Recombinant Human AGFG2 293 Cell Lysate

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Cat.No. : AGFG2-8981HCL
Description : Antigen standard for ArfGAP with FG repeats 2 (AGFG2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AGFG2 ArfGAP with FG repeats 2 [ Homo sapiens ]
Official Symbol : AGFG2
Synonyms : AGFG2; ArfGAP with FG repeats 2; HIV 1 Rev binding protein like , HRBL; arf-GAP domain and FG repeat-containing protein 2; RABR; nucleoporin; HIV-1 Rev-binding protein-like protein; Rev/Rex activation domain binding protein-related; rev/Rex activation domain-binding protein related; arf-GAP domain and FG repeats-containing protein 2; HRBL;
Gene ID : 3268
mRNA Refseq : NM_006076
Protein Refseq : NP_006067
MIM : 604019
UniProt ID : O95081
Chromosome Location : 7q22.1
Function : ARF GTPase activator activity; metal ion binding; molecular_function; zinc ion binding;

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