Recombinant Human ADRA2A 293 Cell Lysate

• Cat.No.: ADRA2A-9000HCL

Specification

• Description: Antigen standard for adrenergic, alpha-2A-, receptor (ADRA2A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADRA2A adrenoceptor alpha3B[ Homo sapiens ]
• Official Symbol: ADRA2A
• Synonyms: ADA2A_HUMAN; ADRA2; ADRA2A; ADRA2R; ADRAR; Adrenergic alpha 2A receptor; Alpha 2 adrenergic receptor subtype C10; Alpha 2A adrenergic receptor; Alpha 2AAR subtype C10; Alpha-2 adrenergic receptor subtype C10; alpha-2-adrenergic receptor, platelet type; Alpha-2A adrenergic receptor; Alpha-2A adrenoceptor; Alpha-2A adrenoreceptor; Alpha-2AAR; ALPHA2AAR; OTTHUMP00000058849; ZNF32
• Gene ID: 150
• mRNA Refseq: NM_000681.3
• Protein Refseq: NP_000672.3
• MIM: 104210
• UniProt ID: P08913
• Chromosome Location: 10q25.2
• Pathway: Adrenaline signalling through Alpha-2 adrenergic receptor;Adrenoceptors;Amine ligand-binding receptor;Class A/1 (Rhodopsin-like receptors);G alpha (i) signalling events;G alpha (z) signalling events;GPCR downstream signaling
• Function: alpha-1B adrenergic receptor binding;alpha-2C adrenergic receptor binding;alpha2-adrenergic receptor activity;;epinephrine binding