Recombinant Human ADCYAP1 293 Cell Lysate

• Cat.No.: ADCYAP1-9019HCL

Specification

• Description: Antigen standard for adenylate cyclase activating polypeptide 1 (pituitary) (ADCYAP1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC210851, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: E, IP, WB. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ADCYAP1 adenylate cyclase activating polypeptide 1 (pituitary) [ Homo sapiens ]
• Official Symbol: ADCYAP1
• Synonyms: ADCYAP1; adenylate cyclase activating polypeptide 1 (pituitary); pituitary adenylate cyclase-activating polypeptide; PACAP; MGC126852
• Gene ID: 116
• mRNA Refseq: NM_001099733
• Protein Refseq: NP_001093203
• MIM: 102980
• UniProt ID: P18509
• Chromosome Location: 18p11
• Pathway: Activation of TRKA receptors, organism-specific biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Glucagon-type ligand receptors, organism-specific biosystem; NGF signalling via TRKA from the plasma membrane, organism-specific biosystem
• Function: neuropeptide hormone activity; peptide hormone receptor binding; peptide hormone receptor binding; receptor binding; receptor signaling protein activity