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Recombinant Human ADAMTS15 293 Cell Lysate

Cat.No. : ADAMTS15-9031HCL
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Description : Antigen standard for ADAM metallopeptidase with thrombospondin type 1 motif, 15 (ADAMTS15) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ADAMTS15 ADAM metallopeptidase with thrombospondin type 1 motif, 15 [ Homo sapiens ]
Official Symbol : ADAMTS15
Synonyms : ADAMTS15; ADAM metallopeptidase with thrombospondin type 1 motif, 15; a disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 15; A disintegrin and metalloproteinase with thrombospondin motifs 15; ADAM-TS15; ADAMTS-15; ADAM-TS 15; metalloprotease disintegrin 15 with thrombospondin domains; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 15, preproprotein; MGC126403;
Gene ID : 170689
mRNA Refseq : NM_139055
Protein Refseq : NP_620686
MIM : 607509
UniProt ID : Q8TE58
Chromosome Location : 11q25
Function : metal ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What are the results of overexpressing ADAMTS15? 09/11/2022

Expression of ADAMTS15 inhibits tumor growth and invasion.

In what tissues is ADAMTS15 mainly expressed? 12/01/2021

ADAMTS15 is mainly expressed in liver and kidney tissues.

Can the ADAMTS15 tag be removed? 07/15/2021

We can remove tags according to your needs.

What is the biological function of ADAMTS15? 04/10/2021

ADAMTS15 has the function of inhibiting tumor growth and vascular synthesis.

Which diseases is ADAMTS15 used to study? 01/02/2021

ADAMTS15 is currently focused on rectal and breast cancer studies.

Does His tag have a great influence on the activity of ADAMTS15? 10/08/2020

Does His tag have a great influence on the activity of ADAMTS15?

Does His tag have a great influence on the activity of ADAMTS15? 09/17/2020

Does His tag have a great influence on the activity of ADAMTS15?

Customer Reviews (4)

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Reviews
01/31/2023

    The technician analyzed my experimental process in detail and offered many constructive suggestions.

    10/17/2022

      The customer service staff was patient and answered many questions for me, which made me get twice the work done.

      03/03/2021

        The company provides a one-stop service, from product purchase to product delivery, the process is very smooth.

        09/22/2020

          A very satisfying shopping experience, whether it is product quality or after-sales service.

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