Recombinant Human ADAM33 293 Cell Lysate


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Recombinant Human ADAM33 293 Cell Lysate

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Cat.No. : ADAM33-9033HCL
Description : Antigen standard for ADAM metallopeptidase domain 33 (ADAM33), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ADAM33 ADAM metallopeptidase domain 33 [ Homo sapiens ]
Official Symbol : ADAM33
Synonyms : ADAM33; ADAM metallopeptidase domain 33; a disintegrin and metalloproteinase domain 33 , C20orf153, chromosome 20 open reading frame 153; disintegrin and metalloproteinase domain-containing protein 33; dJ964F7.1; DKFZp434K0521; ADAM 33; a disintegrin and metalloprotease 33; a disintegrin and metalloproteinase domain 33; disintegrin and reprolysin metalloproteinase family protein; C20orf153; DJ964F7.1; FLJ35308; FLJ36751; MGC71889; MGC149823;
Gene ID : 80332
mRNA Refseq : NM_025220
Protein Refseq : NP_079496
MIM : 607114
UniProt ID : Q9BZ11
Chromosome Location : 20p13
Function : metal ion binding; metalloendopeptidase activity; metalloendopeptidase activity; peptidase activity; zinc ion binding; zinc ion binding;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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