|Cat. No. :
|Product Overview :
||Recombinant Human Formiminotransferase Cyclodeaminase (also called liver cytosol type 1) produced inSf9, is a glycosylated, polypeptide chain having a molecular mass of 59,749 Dalton. The FTCD is expressed with a -6x His tag and purified by proprietary chromatographic techniques.
||Formiminotransferase cyclodeaminase is an enzyme which catalyzes the conversion of formiminoglutamate and tetrahydrofolate into formiminotetrahydrofolate and glutamate. Formiminotransferase cyclodeaminase, a bifunctional enzyme of tetrahydrofolate synthesis, is the target antigen of anti-LC1 (liver cytosol antigen type 1) autoantibodies. Presence of LC1 autoantibodies is a marker for type 2 autoimmune hepatitis (for which anti-LKM 1/cytochrome P450 2D6 autoantibodies are a further marker). Serum LC1 autoantibody concentrations appear to fluctuate in parallel with aminotransferase levels, a particularly intriguing observation that suggests a possible role of LC1 autoreactivity in the pathogenic mechanism leading to hepatocyte injury.
||Sf9 insect cells.
||Greater than 95% as determined by SDS-PAGE.
||FTCD is supplied at a concentration of 0.5 mg/ml in 16mM HEPES buffer pH-7.6, 240 mM sodium chloride, and 20% glycerol.
|Immunological Functions :
||1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).
||Western-Blot with monoclonal anti-hexa-His-tag antibody.and autoimmune hepatitis (AIH II) patient sera.
|Coating Concentration :
||0.35-0.7 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.