Recombinant Human FTCD, His-tagged

• Cat.No.: FTCD-37H
• Product Overview: Recombinant Human Formiminotransferase Cyclodeaminase (also called liver cytosol type 1) produced inSf9, is a glycosylated, polypeptide chain having a molecular mass of 59,749 Dalton. The FTCD is expressed with a -6x His tag and purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: Formiminotransferase cyclodeaminase is an enzyme which catalyzes the conversion of formiminoglutamate and tetrahydrofolate into formiminotetrahydrofolate and glutamate. Formiminotransferase cyclodeaminase, a bifunctional enzyme of tetrahydrofolate synthesis, is the target antigen of anti-LC1 (liver cytosol antigen type 1) autoantibodies. Presence of LC1 autoantibodies is a marker for type 2 autoimmune hepatitis (for which anti-LKM 1/cytochrome P450 2D6 autoantibodies are a further marker). Serum LC1 autoantibody concentrations appear to fluctuate in parallel with aminotransferase levels, a particularly intriguing observation that suggests a possible role of LC1 autoreactivity in the pathogenic mechanism leading to hepatocyte injury.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Formulation: FTCD is supplied at a concentration of 0.5 mg/ml in 16mM HEPES buffer pH-7.6, 240 mM sodium chloride, and 20% glycerol.
• Immunological Functions: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).
• Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody.and autoimmune hepatitis (AIH II) patient sera.
• Coating Concentration: 0.35-0.7 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: FTCD formiminotransferase cyclodeaminase [ Homo sapiens ]
• Synonyms: FTCD; formiminotransferase cyclodeaminase; LCHC1;EC=2.1.2.5;EC=4.3.1.4; Formimidoyltransferase-cyclodeaminase; Glutamate formimidoyltransferase; Glutamate formiminotransferase; Glutamate formyltransferase; Formimidoyltetrahydrofolate cyclodeaminase; human formiminotransferase cyclodeaminase, EC 4.3.1.4; OTTHUMP00000115563
• Gene ID: 10841
• mRNA Refseq: NM_006657
• Protein Refseq: NP_006648
• MIM: 606806
• UniProt ID: O95954
• Chromosome Location: 21q22.3
• Pathway: Histidine metabolism; Metabolic pathways; One carbon pool by folate; Metabolism of amino acids
• Function: folic acid binding; formimidoyltetrahydrofolate cyclodeaminase activity; glutamate formimidoyltransferase activity; lyase activity; transferase activity