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Recombinant Human ACTL6A 293 Cell Lysate

Cat.No. : ACTL6A-9062HCL
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  • Gene Information
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Description : Antigen standard for actin-like 6A (ACTL6A), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ACTL6A actin-like 6A [ Homo sapiens ]
Official Symbol : ACTL6A
Synonyms : ACTL6A; actin-like 6A; actin-like protein 6A; actin related protein 4; Actl6; Arp4; BAF complex 53 kDa subunit; BAF53A; Baf53a; BRG1 associated factor; INO80 complex subunit K; INO80K; BAF53; arpNbeta; hArpN beta; actin-related protein 4; BRG1-associated factor 53A; actin-related protein Baf53a; 53 kDa BRG1-associated factor A; ACTL6; ARPN-BETA;
Gene ID : 86
mRNA Refseq : NM_177989
Protein Refseq : NP_817126
MIM : 604958
UniProt ID : O96019
Chromosome Location : 3q26.33
Pathway : C-MYC pathway, organism-specific biosystem; TNF-alpha/NF-kB Signaling Pathway, organism-specific biosystem; Validated targets of C-MYC transcriptional activation, organism-specific biosystem;
Function : ATP binding; chromatin binding; protein binding; transcription coactivator activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (21)

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How is ACTL6A involved in metastasis and EMT in hepatocellular carcinoma? 08/26/2022

CTL6A plays a critical role in promoting metastasis and epithelial-to-mesenchymal transition (EMT) in hepatocellular carcinoma, processes associated with cancer progression and invasion.

Can ACTL6A be a potential therapeutic target for colon cancer? 04/20/2022

Yes, based on its pro-tumor function and role as an EMT activator in colon cancer, ACTL6A may serve as a potential therapeutic target for developing novel treatment strategies.

Are there any specific molecular pathways or mechanisms associated with ACTL6A's pro-tumor function and EMT activation? 12/10/2021

The specific molecular pathways and mechanisms through which ACTL6A promotes tumor progression and activates EMT in hepatocellular carcinoma and colon cancer are still under investigation.

What is the significance of elevated ACTL6A levels in cancer cells? 07/06/2020

Elevated ACTL6A levels in cancer cells suggest its potential role as a driver in promoting cancer cell survival and tumor formation.

What is the significance of Actl6a acting as a gatekeeper in mESCs? 06/22/2020

Actl6a's role as a gatekeeper in mESCs is to prevent the cells from differentiating into the PrE lineage, ensuring the maintenance of their pluripotency and self-renewal capabilities.

What is the significance of ACTL6A acting as an EMT activator in colon cancer? 03/17/2020

 In colon cancer, ACTL6A functions as an activator of epithelial-to-mesenchymal transition (EMT), a process that contributes to cancer cell invasion, metastasis, and drug resistance.

What is the role of ACTL6A in hepatocellular carcinoma and colon cancer? 08/28/2019

In hepatocellular carcinoma, ACTL6A is involved in metastasis and epithelial-to-mesenchymal transition (EMT). In colon cancer, ACTL6A acts as an EMT activator, exhibiting pro-tumor function.

How does ACTL6A impact embryogenesis and differentiation? 02/22/2019

ACTL6A is vital for embryogenesis and differentiation processes, playing crucial roles in the development of various tissues and organs during embryonic development.

What are the implications of the novel function of Actl6a in mESCs? 10/25/2018

The discovery of Actl6a's role as a gatekeeper in preventing mESCs from entering the PrE lineage expands our understanding of the regulatory mechanisms underlying cell fate decisions in embryonic stem cells.

 In which other tumor types has ACTL6A been identified as a central oncogenic driver? 08/26/2018

ACTL6A has been identified as a central oncogenic driver in various tumor types beyond esophageal squamous cell carcinoma, suggesting its broad significance in cancer development and progression.

What is the potential therapeutic significance of targeting ACTL6A in esophageal squamous cell carcinoma (ESCC)? 07/20/2018

By identifying ACTL6A as a potential therapeutic target, it suggests the possibility of developing alternative treatment strategies specifically for ESCC, potentially leading to improved therapeutic outcomes.

Does ACTL6A have any known functional roles outside of its involvement in chromatin remodeling and tumor development? 07/03/2018

ACTL6A's functional roles beyond its involvement in chromatin remodeling and tumor development are still under investigation.

How does ACTL6A affect p21Cip1 protein levels in aggressive mesothelioma cells? 06/17/2018

ACTL6A suppresses p21Cip1 promoter activity, leading to reduced p21Cip1 protein levels in aggressive mesothelioma cells.

How does Actl6a exhibit a Yin/Yang regulating pattern in mESCs? 05/12/2018

Actl6a demonstrates a Yin/Yang regulating pattern by repressing the expression of PrE regulators while maintaining the expression of pluripotency-related genes, maintaining a balance between differentiation and pluripotency in mESCs.

How do ACTL6A and p63 collaborate as oncogenic drivers in HNSCC? 02/28/2018

ACTL6A and p63 collaborate as oncogenic drivers in HNSCC, suggesting their combined role in promoting the development and progression of this type of cancer.

What are the ATP-dependent chromatin-remodeling complexes SWI/SNF, and how does ACTL6A function within these complexes? 01/01/2018

The SWI/SNF complexes are involved in regulating gene expression by remodeling chromatin structure. ACTL6A acts as a regulatory subunit within these complexes, contributing to their activity and function.

What are the main complexes that Actl6a is a subunit of, and what are their roles in embryonic stem cells? 12/12/2017

Actl6a is a subunit of complexes such as esBAF, INO80, and Tip60-p400, which play crucial roles in maintaining the pluripotency and self-renewal of embryonic stem cells.

How does Actl6a's involvement in multiple complexes contribute to its role in maintaining embryonic stem cells? 11/18/2017

Actl6a's presence in complexes such as esBAF, INO80, and Tip60-p400 suggests its multifaceted involvement in chromatin remodeling and gene regulation, which are crucial for the maintenance of embryonic stem cells.

How does Actl6a impact the expression of key regulators in the primitive endoderm (PrE) lineage? 05/16/2017

Actl6a targets the promoters of key PrE regulators, such as Sall4 and Fgf4, and represses their expression, thereby inhibiting PrE differentiation in embryonic stem cells.

What is the role of ACTL6A in glioblastoma and its potential therapeutic implications? 02/17/2017

The crucial role of ACTL6A in DNA replication and the ATR-Chk1 pathway in glioblastoma, indicating that it could serve as a potential therapeutic target for intervention in this type of cancer.

How does ACTL6A facilitate ESCC cell cycle redistribution through the S6K1/pS6 pathway? 01/16/2016

ACTL6A expression influences ESCC cell cycle redistribution by activating the S6K1/pS6 pathway, which plays a role in regulating cell cycle progression and proliferation.

Customer Reviews (5)

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Reviews
12/06/2021

    Extensive quality control measures, including rigorous testing for activity, stability, and functionality, ensure that the protein product meets the highest standards of quality.

    10/25/2021

      Stringent quality control protocols are followed during the production process, ensuring the protein's consistent performance and characteristics.

      12/19/2017

        The company's customer support team is highly responsive and knowledgeable, promptly addressing any inquiries or concerns we have regarding the protein product.

        09/18/2016

          We have observed significant improvements in experimental outcomes

          05/16/2016

            We have consistently received our orders on time, with efficient shipping and reliable tracking information provided by the company.

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