Recombinant Human ACSM5 293 Cell Lysate

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Recombinant Human ACSM5 293 Cell Lysate

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Cat.No. : ACSM5-9069HCL
Description : Antigen standard for acyl-CoA synthetase medium-chain family member 5 (ACSM5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ACSM5 acyl-CoA synthetase medium-chain family member 5 [ Homo sapiens ]
Official Symbol : ACSM5
Synonyms : ACSM5; acyl-CoA synthetase medium-chain family member 5; acyl-coenzyme A synthetase ACSM5, mitochondrial; FLJ20581;
Gene ID : 54988
mRNA Refseq : NM_017888
Protein Refseq : NP_060358
MIM : 614361
UniProt ID : Q6NUN0
Chromosome Location : 16p12.3
Pathway : Butanoate metabolism, organism-specific biosystem; Butanoate metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem;
Function : ATP binding; GTP binding; butyrate-CoA ligase activity; ligase activity; metal ion binding; nucleotide binding;

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