Recombinant Human ACSF2 293 Cell Lysate

• Cat.No.: ACSF2-9078HCL

Specification

• Description: Antigen standard for acyl-CoA synthetase family member 2 (ACSF2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACSF2 acyl-CoA synthetase family member 2 [ Homo sapiens ]
• Official Symbol: ACSF2
• Synonyms: ACSF2; acyl-CoA synthetase family member 2; acyl-CoA synthetase family member 2, mitochondrial; ACSMW; FLJ20920; PPARG binding, long chain fatty acid acyl Co-A ligase like; AVYV493
• Gene ID: 80221
• mRNA Refseq: NM_025149
• Protein Refseq: NP_079425
• MIM: 610465
• UniProt ID: Q96CM8
• Chromosome Location: 17q21.33
• Function: ATP binding; ligase activity; nucleotide binding

Reviews

10/07/2021

Super fast shipping and excellent customer service. Will definitely buy from again.

09/14/2021

Great product! Really satisfied with the quality of the recombinant protein.

07/13/2020

Wonderful product!

09/25/2018

Overall, I highly recommend this recombinant protein and the manufacturer for their excellent product and service.

08/21/2018

I was impressed with the strict quality control measures the manufacturer had in place to ensure consistent quality.

Q&As

Are there any therapeutic implications of targeting ACSF2?

ACSF2 inhibitors have been proposed as a potential therapeutic strategy for the treatment of metabolic disorders, although further research is needed to validate this approach.

What is the substrate specificity of ACSF2?

ACSF2 has a broad substrate specificity and can activate a range of long-chain fatty acids including palmitic acid, oleic acid, and arachidonic acid.

What is the mechanism of ACSF2-mediated fatty acid activation?

ACSF2 activates fatty acids by converting them into their acyl-CoA derivatives through the use of ATP and CoA.

What is the role of ACSF2 in cellular stress response?

ACSF2 has been shown to play a role in the cellular stress response by regulating the activation of AMPK and the expression of stress response genes.

What is the role of ACSF2 in cancer?

ACSF2 has been shown to play a role in tumor growth and progression in several types of cancer, including colorectal cancer and hepatocellular carcinoma.

Where is ACSF2 primarily expressed?

ACSF2 is highly expressed in the liver and also in other tissues such as the brain, heart, and muscle.

How does ACSF2 affect lipid droplet dynamics?

ACSF2 has been shown to play a role in the regulation of lipid droplet dynamics by regulating the activity of lipases and promoting the formation of lipid droplets.

What is the effect of ACSF2 overexpression on fatty acid metabolism?

Overexpression of ACSF2 has been shown to increase fatty acid oxidation and decrease fatty acid synthesis in hepatocytes.

How does ACSF2 affect insulin sensitivity?

ACSF2 has been shown to play a role in insulin sensitivity by regulating glucose uptake and lipid metabolism in skeletal muscle cells.

How is ACSF2 involved in the regulation of inflammation?

ACSF2 has been shown to regulate the production of inflammatory cytokines in macrophages and may play a role in the development of inflammatory diseases.