Recombinant Human ACOX2 293 Cell Lysate

• Cat.No.: ACOX2-9085HCL

Specification

• Description: Antigen standard for acyl-Coenzyme A oxidase 2, branched chain (ACOX2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ACOX2 acyl-CoA oxidase 2, branched chain [ Homo sapiens ]
• Official Symbol: ACOX2
• Synonyms: ACOX2; acyl-CoA oxidase 2, branched chain; acyl Coenzyme A oxidase 2, branched chain; peroxisomal acyl-coenzyme A oxidase 2; BRCACOX; BRCOX; THCA-CoA oxidase; trihydroxycoprostanoyl-CoA oxidase; acyl-Coenzyme A oxidase 2, branched chain; peroxisomal branched chain acyl-CoA oxidase; 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA oxidase; 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA 24-hydroxylase; BCOX; THCCox
• Gene ID: 8309
• mRNA Refseq: NM_003500
• Protein Refseq: NP_003491
• MIM: 601641
• UniProt ID: Q99424
• Chromosome Location: 3p14.3
• Pathway: Beta-oxidation of pristanoyl-CoA, organism-specific biosystem; Bile acid and bile salt metabolism, organism-specific biosystem; Bile acid biosynthesis, cholesterol =>cholate, organism-specific biosystem; Bile acid biosynthesis, cholesterol => cholate, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA 24-hydroxylase activity; acyl-CoA dehydrogenase activity; acyl-CoA oxidase activity; fatty acid binding; flavin adenine dinucleotide binding; pristanoyl-CoA oxidase activity