"ACOT7" Related Products


Recombinant Human ACOT7 293 Cell Lysate

Cat.No.: ACOT7-9087HCL
Description: Antigen standard for acyl-CoA thioesterase 7 (ACOT7), transcript variant hBACHd is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ACOT7 acyl-CoA thioesterase 7 [ Homo sapiens ]
Official Symbol: ACOT7
Synonyms: ACOT7; acyl-CoA thioesterase 7; cytosolic acyl coenzyme A thioester hydrolase; ACH1; ACT; BACH; brain acyl CoA hydrolase; CTE II; hBACH; LACH1; MGC1126; CTE-IIa; acyl-CoA thioesterase 2; brain acyl-CoA hydrolase; acyl-CoA thioesterase, long chain; long chain acyl-CoA thioester hydrolase; LACH; CTE-II; RP1-120G22.10;
Gene ID: 11332
mRNA Refseq: NM_007274
Protein Refseq: NP_009205
MIM: 602587
UniProt ID: O00154
Chromosome Location: 1p36
Pathway: Biosynthesis of unsaturated fatty acids, organism-specific biosystem; Biosynthesis of unsaturated fatty acids, conserved biosystem; Fatty acid elongation, organism-specific biosystem; Fatty acid elongation, conserved biosystem;
Function: carboxylesterase activity; catalytic activity; fatty-acyl-CoA binding; hydrolase activity; palmitoyl-CoA hydrolase activity;

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