Recombinant Human ACOT12 293 Cell Lysate


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Recombinant Human ACOT12 293 Cell Lysate

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Cat.No. : ACOT12-9090HCL
Description : Antigen standard for acyl-CoA thioesterase 12 (ACOT12) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ACOT12 acyl-CoA thioesterase 12 [ Homo sapiens ]
Official Symbol : ACOT12
Synonyms : ACOT12; acyl-CoA thioesterase 12; acyl-coenzyme A thioesterase 12; Cach; StAR related lipid transfer (START) domain containing 15; STARD15; THEAL; hCACH-1; cytosolic acetyl-CoA hydrolase; acyl-CoA thioester hydrolase 12; START domain-containing protein 15; cytoplasmic acetyl-CoA hydrolase 1; StAR-related lipid transfer (START) domain containing 15; CACH-1; MGC105114;
Gene ID : 134526
mRNA Refseq : NM_130767
Protein Refseq : NP_570123
MIM : 614315
UniProt ID : Q8WYK0
Chromosome Location : 5q14.1
Pathway : Pyruvate metabolism, organism-specific biosystem; Pyruvate metabolism, conserved biosystem;
Function : ATP binding; acetyl-CoA hydrolase activity; carboxylesterase activity; hydrolase activity;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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