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Recombinant Human ACD 293 Cell Lysate

Cat.No.: ACD-9097HCL
Description: Antigen standard for adrenocortical dysplasia homolog (mouse) (ACD), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ACD adrenocortical dysplasia homolog (mouse) [ Homo sapiens ]
Official Symbol: ACD
Synonyms: ACD; adrenocortical dysplasia homolog (mouse); adrenocortical dysplasia protein homolog; Pip1; POT1 and TIN2 organizing protein; Ptop; TIN2 interacting protein 1; Tint1; Tpp1; POT1 and TIN2-interacting protein; PIP1; PTOP; TPP1; TINT1;
Gene ID: 65057
mRNA Refseq: NM_022914
Protein Refseq: NP_075065
MIM: 609377
UniProt ID: Q96AP0
Chromosome Location: 16q22
Pathway: Cell Cycle, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; Meiosis, organism-specific biosystem; Meiotic Synapsis, organism-specific biosystem; Packaging Of Telomere Ends, organism-specific biosystem; Regulation of Telomerase, organism-specific biosystem; Shelterin complex, organism-specific biosystem;
Function: DNA binding; DNA polymerase binding; protein binding;

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