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Recombinant Human ACADS 293 Cell Lysate

Cat.No.: ACADS-9113HCL
Description: Antigen standard for acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain (ACADS), nuclear gene encoding mitochondrial protein is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ACADS acyl-CoA dehydrogenase, C-2 to C-3 short chain [ Homo sapiens ]
Official Symbol: ACADS
Synonyms: ACADS; acyl-CoA dehydrogenase, C-2 to C-3 short chain; acyl Coenzyme A dehydrogenase, C 2 to C 3 short chain; short-chain specific acyl-CoA dehydrogenase, mitochondrial; ACAD3; SCAD; butyryl-CoA dehydrogenase; unsaturated acyl-CoA reductase; short-chain acyl-CoA dehydrogenase; acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain; mitochondrial short-chain specific acyl-CoA dehydrogenase;
Gene ID: 35
mRNA Refseq: NM_000017
Protein Refseq: NP_000008
MIM: 606885
UniProt ID: P16219
Chromosome Location: 12q24.31
Pathway: Beta oxidation of butanoyl-CoA to acetyl-CoA, organism-specific biosystem; Beta oxidation of hexanoyl-CoA to butanoyl-CoA, organism-specific biosystem; Butanoate metabolism, organism-specific biosystem; Butanoate metabolism, conserved biosystem; Fatty Acid Beta Oxidation, organism-specific biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem;
Function: acyl-CoA dehydrogenase activity; butyryl-CoA dehydrogenase activity; fatty-acyl-CoA binding; flavin adenine dinucleotide binding;

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