Recombinant Human Kallikrein 1

• Cat.No.: KLK1-38H
• Product Overview: Recombinant Human KLK1 produced in Yeast is a single, glycosylated, polypeptide chain containing 238 amino acids and having a molecular mass of 28-32 kDa. KLK1 Human Recombinant is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: P.pastoris
• Tag: Non
• Description: KLK1 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. KLK1 is functionally conserved in its ability to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen. Human Kallikrein-1, also called as Kallidinogenase, Kininogenase or Kininogenin, is an active protein enzyme present in saliva, pancreatic juices, and urine that catalyzes the proteolysis of bradykininogen to bradykinin. Kallikrein-1, which derived from human or porcine, have been used as drugs for a long time, they are mainly used in the treatment f light to medium hypertension and occlusion of cerebral and surrounding blood vessels. KLK1 demonstrates both trypsin- and chymotrypsin-like selectivities with Tyr/Arg preferred at site P1, Ser/Arg strongly preferred at P1", and Phe/Leu at P2.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ile-Val-Gly-Gly-Trp-Glu.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 98.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein solution contains 1X PBS.
• Biological Activity: Human Kallikrein-1 active Unit Definitin: 1 unit will hydrolize 1 μmole of H-D-Val-Leu-Arg-pNA(S-2266) per minute at pH 8.0 at 37°C Human Kallikrein-1 measured biological activity of no less than 5 Units/mg (S2266 method).
• Stability: KLK1 although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
• Publications:
  • Kallikrein–Kinin system suppresses Type i interferon responses: a novel Pathway of interferon regulation (2018)

Gene Information

• Gene Name: KLK1 kallikrein 1 [ Homo sapiens ]
• Synonyms: kallikrein serine protease 1; kallikrein 1, renal/pancreas/salivary; Klk6; KLKR; KLK1; hK1; kallikrein 1; tissue kallikrein; glandular kallikrein 1; EC 3.4.21.35; Kidney/pancreas/salivary gland kallikrein
• Gene ID: 3816
• mRNA Refseq: NM_002257
• Protein Refseq: NP_002248
• MIM: 147910
• UniProt ID: P06870
• Chromosome Location: 19q13.3
• Function: peptidase activity; serine-type endopeptidase activity