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Recombinant Human GPD2 cell lysate

Cat.No.: GPD2-733HCL
Description: Mitochondrial glycerophosphate dehydrogenase (EC 1.1.99.5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.
Species: Human
Size: 100 ug
Recommended Usage: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer: In modified RIPA Lysis Buffer.
Applications: Western Blot;Immunoprecipitation;
Gene Name: GPD2 glycerol-3-phosphate dehydrogenase 2 (mitochondrial) [ Homo sapiens ]
Official Symbol: GPD2
Synonyms: GPD2; glycerol-3-phosphate dehydrogenase 2 (mitochondrial); glycerol-3-phosphate dehydrogenase, mitochondrial; GPD-M; mtGPD; GPDH-M; mitochondrial glycerophosphate dehydrogenase; GDH2; GPDM; mGPDH;
Gene ID: 2820
mRNA Refseq: NM_000408
Protein Refseq: NP_000399
MIM: 138430
UniProt ID: P43304
Chromosome Location: 2q24.1
Pathway: Fatty Acid Beta Oxidation, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; glycerol-3-phosphate shuttle, organism-specific biosystem;
Function: calcium ion binding; glycerol-3-phosphate dehydrogenase [NAD+] activity; glycerol-3-phosphate dehydrogenase activity; oxidoreductase activity; sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity;

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