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Recombinant Human Alanyl-tRNA Synthetase, His-tagged

Cat. No.: AARS-51H
Product Overview: Recombinant HumanAlanyl-tRNA synthetase produced inSf9is a glycosylated, polypeptide chain having a molecular mass of 107,598 Dalton. PL-12 is expressed with a -6x His tag and purified by proprietary chromatographic techniques.
Description: Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108 kDa protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop.
Source: Sf9 insect cells.
Physical Appearance: Sterile Filtered clear solution.
Purity: Greater than 90% as determined by SDS-PAGE.
Formulation: PL-12 is supplied at a of 0.9 mg/ml in16 mM HEPES buffer pH-8, 250 mM sodium chloride, and 20% glycerol.
Immunological Functions: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).
Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.
Coating Concentration: 0.3-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Gene Name: AARS alanyl-tRNA synthetase [ Homo sapiens ]
Synonyms: AARS; alanyl-tRNA synthetase; alanine tRNA ligase 1, cytoplasmic; Alanyl-tRNA synthetase, cytoplasmic; Renal carcinoma antigen NY-REN-42; Alanine--tRNA ligase; AlaRS; EC 6.1.1.7
Gene ID: 16
mRNA Refseq: NM_001605
Protein Refseq: NP_001596
MIM: 601065
UniProt ID: P49588
Chromosome Location: 16q22
Pathway: Aminoacyl-tRNA biosynthesis; Gene Expression
Function: ATP binding; alanine-tRNA ligase activity; ligase activity; nucleic acid binding; tRNA binding

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