PtK2 (Marsupial kidney) whole cell lysate PtK2-1434M

Cat.No.:	PtK2-1434M
Species:	Marsupials
Tissue Type:	Marsupial Kidney; Normal
Preparation method:	Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml.
Size:	200 ug
Recommended Usage:	Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer:	In modified RIPA Lysis Buffer.
Applications:	Western Blot;