Recombinant Human DAAM1 cell lysate

• Cat.No.: DAAM1-2111HCL

Specification

• Description: Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.
• Species: Human
• Size: 100 ug
• Recommended Usage: Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Storage Buffer: In modified RIPA Lysis Buffer.
• Applications: Western Blot;Immunoprecipitation

Gene Information

• Gene Name: DAAM1 dishevelled associated activator of morphogenesis 1 [ Homo sapiens ]
• Official Symbol: DAAM1
• Synonyms: DAAM1; dishevelled associated activator of morphogenesis 1; disheveled-associated activator of morphogenesis 1; KIAA0666; FLJ41657
• Gene ID: 23002
• mRNA Refseq: NM_014992
• Protein Refseq: NP_055807
• MIM: 606626
• UniProt ID: Q9Y4D1
• Chromosome Location: 14q22.3
• Pathway: Noncanonical Wnt signaling pathway, organism-specific biosystem; Wnt Signaling Pathway NetPath, organism-specific biosystem; Wnt signaling pathway, organism-specific biosystem; Wnt signaling pathway, conserved biosystem
• Function: Rho GTPase binding; actin binding; binding