Active Recombinant Human CD3E&CD3D, His-Fc&Flag-Fc tagged
Cat.No. : | CD3E&CD3D-182H |
Product Overview : | Recombinant Human CD3E & CD3D heterodimer protein Fc Chimera (Human CD3E & CD3D heterodimer Fc Chimera) Asp 23 - Asp 126 (CD3E, NP_000724.1) and & Phe 22 - Ala 105 (CD3D, AAH39035) was produced in human 293 cells (HEK293). |
- Specification
- Gene Information
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Description : | T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. |
Source : | HEK293 |
Species : | Human |
Tag : | His-Fc&Flag-Fc |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized Human CD3E & CD3D heterodimer Fc Chimera at 10 μg/mL (100 μL/well) can bind OKT3-mIgG2a (CD3E MAb) with a linear ranger of 3.4-50 ng/mL. |
Molecular Mass : | The heterodimer human CD3E & CD3D Fc Chimera is produced by co-expression of CD3E and CD3D, has a calculated MW of 39.2 kDa (CD3E), 37.5 kDa (CD3D) . Subunit CD3E is fused with his-tagged human IgG1 Fc fragment at the C-terminus and subunit CD3D is fused |
Endotoxin : | Less than 1.0 EU per μg of the Human CD3E & CD3D Heterodimer Fc Chimera by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC). |
Reconstitution : | See Certificate of Analysis for reconstitution instructions and specific concentrations. |
Gene Name : | CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ] |
Official Symbol : | CD3E |
Synonyms : | CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683; |
Gene ID : | 916 |
mRNA Refseq : | NM_000733 |
Protein Refseq : | NP_000724 |
UniProt ID : | P07766 |
Chromosome Location : | 11q23 |
Pathway : | Adaptive Immune System, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Downstream TCR signaling, organism-specific biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem; |
Function : | SH3 domain binding; T cell receptor binding; protein heterodimerization activity; protein kinase binding; receptor activity; receptor signaling complex scaffold activity; receptor signaling protein activity; transmembrane signaling receptor activity; |
Products Types
◆ Recombinant Protein | ||
CD3E-141H | Recombinant Human CD3E Protein, His-tagged | +Inquiry |
Cd3e-839M | Recombinant Mouse Cd3e Protein, MYC/DDK-tagged | +Inquiry |
CD3E-140C | Recombinant Cynomolgus monkey CD3E Protein, His and Fc-tagged | +Inquiry |
Cd3e-322R | Recombinant Rhesus CD3E Protein, His-tagged | +Inquiry |
CD3E-676H | Recombinant Human CD3E Protein, His and C-hFc-tagged | +Inquiry |
◆ Lysates | ||
CD3E-1602CCL | Recombinant Cynomolgus CD3E cell lysate | +Inquiry |
CD3E-1275HCL | Recombinant Human CD3E cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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