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Recombinant Human CD3E&CD3D, His-Fc&Flag-Fc tagged

Cat.No.: CD3E&CD3D-182H
Product Overview: Recombinant Human CD3E & CD3D heterodimer protein Fc Chimera (Human CD3E & CD3D heterodimer Fc Chimera) Asp 23 - Asp 126 (CD3E, NP_000724.1) and & Phe 22 - Ala 105 (CD3D, AAH39035) was produced in human 293 cells (HEK293).
Description: T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Source: HEK293
Species: Human
Tag: His-Fc&Flag-Fc
Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD3E & CD3D heterodimer Fc Chimera at 10 μg/mL (100 μL/well) can bind OKT3-mIgG2a (CD3E MAb) with a linear ranger of 3.4-50 ng/mL.
Molecular Mass: The heterodimer human CD3E & CD3D Fc Chimera is produced by co-expression of CD3E and CD3D, has a calculated MW of 39.2 kDa (CD3E), 37.5 kDa (CD3D) . Subunit CD3E is fused with his-tagged human IgG1 Fc fragment at the C-terminus and subunit CD3D is fused
Endotoxin: Less than 1.0 EU per μg of the Human CD3E & CD3D Heterodimer Fc Chimera by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.
Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
Official Symbol: CD3E
Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683;
Gene ID: 916
mRNA Refseq: NM_000733
Protein Refseq: NP_000724
UniProt ID: P07766
Chromosome Location: 11q23
Pathway: Adaptive Immune System, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Downstream TCR signaling, organism-specific biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem;
Function: SH3 domain binding; T cell receptor binding; protein heterodimerization activity; protein kinase binding; receptor activity; receptor signaling complex scaffold activity; receptor signaling protein activity; transmembrane signaling receptor activity;

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