Recombinant Mouse CD3E & CD3D protein, hFc-tagged

• Cat.No.: CD3E & CD3D-401M
• Product Overview: Recombinant Mouse CD3E&CD3D Protein is expressed from mammalian with a hFc Tag at the C-terminal It contains Asp23-Asp108 (P22646) & Phe22-Ala105 (P04235).

Specification

• Description: T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
• Source: HEK293
• Species: Mouse
• Tag: hFc
• Form: Supplied as 0. 22um filtered solution in PBS (pH 7. 4).
• Molecular Mass: The protein has a predicted MW of 36. 1 kDa (CD3E )and 35. 2 kDa (CD3D ). Due to glycosylation, the protein migrates to 50-65KDa and 40-50KDa based on Bis-Tris PAGE result.
• Protein length: It contains Asp23-Asp108 (P22646) & Phe22-Ala105 (P04235).
• Endotoxin: Less than 0. 1 EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Cd3e CD3 antigen, epsilon polypeptide [ Mus musculus ]
• Official Symbol: CD3E; CD3D
• Synonyms: CD3;CD3D;CD3-DELTA;T3D;CD3E
• Gene ID: 12501
• mRNA Refseq: NM_007648
• Protein Refseq: NP_031674
• UniProt ID: P22646