Active Recombinant Mouse CD3E & CD3D protein, hFc-tagged, Low Endotoxin

• Cat.No.: CD3E & CD3D-401M
• Product Overview: Active Recombinant Mouse CD3E & CD3D protein(P22646(CD3E) & P04235(CD3D))(Asp23-Asp108(CD3E) and Phe22-Ala105(CD3D)) is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 23-108 aa(CD3E) & 22-105 aa(CD3D)
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Mouse CD3E&CD3D, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD3E Antibody (145-2C11), mFc Tag with the EC50 of 23.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 36.1 kDa (CD3E) and 35.2 kDa (CD3D). Due to glycosylation,the protein migrates to 50-65 kDa and 40-50 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Cd3e CD3 antigen, epsilon polypeptide [ Mus musculus ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3 antigen, epsilon polypeptide; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3; T3e; AI504783; CD3epsilon
• Gene ID: 12501
• mRNA Refseq: NM_007648
• Protein Refseq: NP_031674
• Gene Name 2: Cd3d CD3 antigen, delta polypeptide [ Mus musculus ]
• Official Symbol 2: CD3D
• Synonyms 2: CD3D; CD3 antigen, delta polypeptide; T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; T3d
• Gene ID 2: 12500
• mRNA Refseq 2: NM_013487
• Protein Refseq 2: NP_038515