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Recombinant Human CFD, His-tagged

Cat.No.: CFD-187H
Product Overview: Recombinant Human CFD /Adipsin Protein (rh CFD / Adipsin) Ile 26 - Ala 253 (Accession # AAH57807) was produced in human 293 cells (HEK293).
Description: Complement factor D (CFD) is also known as Adipsin, C3 convertase activator, Properdin factor D (PFD), which contains one peptidase S1 domain and belongs to the peptidase S1 family. CFD / Adipsin cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. CFD / Adipsin is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD / Adipsin are the cause of complement factor D deficiency which predisposes to invasive meningococcal disease.
Source: HEK293
Species: Human
Tag: His
Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5"Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >70 pmol/min/μg.
Molecular Mass: rh CFD / Adipsin is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 25.2 kDa. The predicted N-terminus is Ile 26. DTT-reduced Protein migrates as 25 kDa in SDS-PAGE.
Endotoxin: Less than 1.0 EU per μg of the rh CFD / Adipsin by the LAL method.
Purity: >90% as determined by SDS-PAGE.
Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.
Gene Name: CFD complement factor D (adipsin) [ Homo sapiens ]
Official Symbol: CFD
Synonyms: CFD; complement factor D (adipsin); D component of complement (adipsin) , DF, PFD, properdin factor D; complement factor D; ADN; properdin factor D; C3 convertase activator; complement factor D preproprotein; D component of complement (adipsin); DF; PFD; ADIPSIN;
Gene ID: 1675
mRNA Refseq: NM_001928
Protein Refseq: NP_001919
MIM: 134350
UniProt ID: P00746
Chromosome Location: 19p13.3
Pathway: Adipogenesis, organism-specific biosystem; Alternative complement activation, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Hemostasis, organism-specific biosystem; Immune System, organism-specific biosystem;
Function: peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity;

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