Active Recombinant Human PDK2, GST-tagged

• Cat.No.: PDK2-1071H
• Product Overview: Recombinant full-length human PDHK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: Sf9 insect cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 7.5 nmol/min/mg
• Molecular Mass: ~70 kDa
• Protein length: Full Length
• Purity: >95%
• Applications: Kinase Assay; Western Blot
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: PDK2 pyruvate dehydrogenase kinase, isozyme 2 [ Homo sapiens ]
• Official Symbol: PDK2
• Synonyms: PDK2; pyruvate dehydrogenase kinase, isozyme 2; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial; pyruvate dehydrogenase kinase, isoenzyme 2; pyruvate dehydrogenase, lipoamide, kinase isozyme 2, mitochondrial; PDHK2; EC 2.7.11.2
• Gene ID: 5164
• mRNA Refseq: NM_002611
• Protein Refseq: NP_002602
• MIM: 602525
• UniProt ID: Q15119
• Chromosome Location: 17q21.33
• Pathway: B Cell Receptor Signaling Pathway; Pyruvate metabolism; Regulation of pyruvate dehydrogenase (PDH) complex
• Function: ATP binding; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity