Active Recombinant Human WHSC1, FLAG-tagged

• Cat.No.: WHSC1-57H
• Product Overview: Recombinant Human WHSC1 protein (accession number NP_579877.1) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 154 kDa.

Specification

• Description: MMSET (Multiple Myeloma SET domain-containing protein) / WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1; also known as NSD2, Nuclear Receptor binding SET domain protein 2; TRX5, Trithorax 5) is a SET domain-containing histone methyltransferase specific for lysine 36 of histone H3. The MMSET / WHSC1 gene is deleted in Wolf-Hirschhorn syndrome, a disorder characterized by developmental defects and mental retardation. In 15% of patients with Multiple Myeloma (MM), a chromosomal translocation leads to MMSET / WHSC1 gene being aberrantly placed under the control of the IgH intronic μ enhancer and expressed at very high levels. This event is apparently a key component in cellular transformation leading to MM. Reduction in MMSET / WHSC1 expression in a myeloma cell line suppressed its growth, suggesting a role for MMSET in maintaining the transformed state.
• Source: Sf9 insect cells
• Species: Human
• Tag: FLAG
• Form: 25 mM HEPES, pH 7.5, 150 mM NaCl and 5% glycerol.
• Bio-activity: Histone methyltransferase with histone H3 "Lys-27" (H3K27me) methyltransferase activity.
• Applications: Enzyme kinetics, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: WHSC1 Wolf-Hirschhorn syndrome candidate 1 [ Homo sapiens ]
• Official Symbol: WHSC1
• Synonyms: WHSC1; Wolf-Hirschhorn syndrome candidate 1; probable histone-lysine N-methyltransferase NSD2; MMSET; NSD2; trithorax/ash1-related protein 5; nuclear SET domain-containing protein 2; IL5 promoter REII region-binding protein; multiple myeloma SET domain containing protein type III; WHS; TRX5; REIIBP; FLJ23286; KIAA1090; MGC176638
• Gene ID: 7468
• mRNA Refseq: NM_001042424
• Protein Refseq: NP_001035889
• MIM: 602952
• UniProt ID: O96028
• Chromosome Location: 4p16.3
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem
• Function: DNA binding; histone-lysine N-methyltransferase activity; metal ion binding; methyltransferase activity; transferase activity; zinc ion binding