Recombinant Human FOXA1

• Cat.No.: FOXA1-126H
• Product Overview: Recombinant Human FOXA1 was expressed in E. coli and refolded to soluble form.

Specification

• Description: FOXA1 is a member of the forkhead box family of DNA-binding proteins. FOXA1 is a hepatocyte nuclear factor (specifically, HNF-3α) that is a transcriptional activator for liver-specific genes such as AFP and albumin. During embryonic development, FoxA1 is involved in the development of multiple endoderm-derived organs such as liver, pancreas, lung and prostate. In studying in vitro stem cell differentiation, FOXA1 expression is used as a marker for endoderm lineage cells.
• Source: E. coli
• Species: Human
• Form: 200 µg/ml (determined by Bradford assay) in 500 mM L-arginine, 300 mM guanidine-HCl, 50 mM HEPES, 5 mM cystamine, 5 mM cysteamine, 5 mM CaCl2, 3 mM DTT, pH 7.5.
• Molecular Mass: 472 AA; 49.1 kDa
• Purity: >80% by SDS-PAGE
• Storage: Store at -80 °C. Avoid multiple freeze-thaws.

Gene Information

• Gene Name: FOXA1 forkhead box A1 [ Homo sapiens ]
• Official Symbol: FOXA1
• Synonyms: FOXA1; forkhead box A1; hepatocyte nuclear factor 3, alpha , HNF3A; hepatocyte nuclear factor 3-alpha; HNF-3A; TCF-3A; HNF-3-alpha; forkhead box protein A1; transcription factor 3A; hepatocyte nuclear factor 3, alpha; HNF3A; TCF3A; MGC33105
• Gene ID: 3169
• mRNA Refseq: NM_004496
• Protein Refseq: NP_004487
• MIM: 602294
• UniProt ID: P55317
• Chromosome Location: 14q12-q13
• Pathway: Direct p53 effectors, organism-specific biosystem; FOXA transcription factor networks, organism-specific biosystem; FOXA1 transcription factor network, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem
• Function: DNA binding; DNA binding, bending; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; double-stranded DNA binding; protein domain specific binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription factor binding; transcription regulatory region DNA binding