||Recombinant human IDOL (amino acid residues 1-445), with N-terminal 6His, was expressed in E.coli.
||The enzymes of the ubiquitylation pathway play a pivotal role in a number of cellular processes including the regulated and targeted proteasome-dependent degradation of substrate proteins. Three classes of enzymes are involved in the process of ubiquitylation; activating enzymes (E1s), conjugating enzymes (E2s) and protein ligases (E3s).
||50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
||12 months at -70°C. Avoid multiple freeze/thaw cycles.