Active Recombinant Human PRKAA2/PRKAB2/PRKAG1, His-tagged

• Cat.No.: PRKAA2-145H
• Product Overview: Recombinant human PRKAA2 (amino acid residues 2-552), with N-terminal 6His, PRKAB2 (amino acid residues 1-272), PRKAG1 (amino acid residues 1-331) was expressed in E.coli.

Specification

• Description: Cloning of human 5"-AMP-activated protein kinase subunits alpha, beta and gamma (AMPKαβγ) was first described by Stapleton et al. (1996; 1997). AMPK is a highly conserved heterotrimeric enzyme consisting of three subunits, α, β, and γ, with multiple genes encoding distinct subunit isoforms (ie, α1, α2, β1, β2, γ1, γ2, and γ3) (Zungu et al., 2011).
• Source: E. coli
• Species: Human
• Tag: His
• Form: 50 mM Tris/HCl pH7.5, 0.1 mM EGTA, 150 mM NaCl, 0.1% ?-Mercaptoethanol, 270 mM sucrose, 0.03% Brij-35, 1 mM Benzamidine, 0.2 mM PMSF.
• Bio-activity: 4435 Units/mg (1198 Units/ml) Substrate: AMARA peptide (AMARAASAAALARRR)
• Molecular Mass: AMPKA2 ~63.2 kDa; AMPKB2 ~30.3 kDa; AMPKG1 ~37.6 kDa
• Unit Definition: 1 Unit = 1 nmole of phosphate incorporated into the substrate in 1 minute.
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.27 mg/ml

Gene Information

• Gene Name: PRKAA2 protein kinase, AMP-activated, alpha 2 catalytic subunit [ Homo sapiens ]
• Official Symbol: PRKAA2
• Synonyms: PRKAA2; protein kinase, AMP-activated, alpha 2 catalytic subunit; PRKAA; 5-AMP-activated protein kinase catalytic subunit alpha-2; AMPK; AMPKa2; AMPK-alpha-2 chain; AMPK subunit alpha-2; 5-AMP-activated protein kinase, catalytic alpha-2 chain; AMPK2
• Gene ID: 5563
• mRNA Refseq: NM_006252
• Protein Refseq: NP_006243
• MIM: 600497
• UniProt ID: P54646
• Chromosome Location: 1p31
• Pathway: AMPK inhibits chREBP transcriptional activation activity, organism-specific biosystem; AMPK signaling, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Energy Metabolism, organism-specific biosystem; Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem
• Function: AMP-activated protein kinase activity; AMP-activated protein kinase activity; ATP binding; chromatin binding; histone serine kinase activity; metal ion binding; nucleotide binding; protein binding; protein binding, bridging; protein kinase activity; protein serine/threonine kinase activity