Active Recombinant Human CUL5/RNF7

• Cat.No.: CUL5-149H
• Product Overview: Recombinant human CUL5 (amino acid residues 1-780), Rnf7 (amino acid residues 1-113) was expressed in Insect (Sf21) cells.

Specification

• Description: Cullin-RING-Ligases (CRLs) are one largest class of ubiquitin E3 ligases and the enzymes of the NEDDylation pathway play a pivotal role in the activation of these, akin to ubiquitylation, the E1 activating enzyme (APP-BP1/UBA3 heterodimer) and the E2 conjugating enzymes (UBE2M or UBE2F) are involved in mammalian NEDDylation of the Cullin Ring Ligases (CRLs) (Meyer-Schaller et al., 2009; Huang et al., 2011; Morimoto et al., 2003).
• Source: Insect cells
• Species: Human
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: E3 Ligase Assay: The activity of Cul5/Rnf7 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase His-DCNL1 and thioester loaded His-Ube2M-NEDD8. Incubation of Cul5/Rnf7 and thioester loaded His-Ube2M-NEDD8 in the presence or absence of His-DCNL1 at 4?C was compared at two time points T0 and T2 minutes. Increased Neddylation of the Cul5 subunit in the presence of His-DCNL1 was demonstrated.
• Molecular Mass: Cul5=121kDa; Rnf7=13kDa
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: CUL5 cullin 5 [ Homo sapiens ]
• Official Symbol: CUL5
• Synonyms: CUL5; cullin 5; cullin-5; VACM 1; CUL-5; Vasopressin-activated calcium-mobilizing receptor-1; vasopressin-activated calcium-mobilizing receptor 1; Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1); VACM1; VACM-1
• Gene ID: 8065
• mRNA Refseq: NM_003478
• Protein Refseq: NP_003469
• MIM: 601741
• UniProt ID: Q93034
• Chromosome Location: 11q22.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Disease, organism-specific biosystem; ECS complex, organism-specific biosystem
• Function: calcium channel activity; protein binding; receptor activity; ubiquitin protein ligase binding