Recombinant Human CUL5/RNF7 CUL5-149H

Recombinant Human CUL5/RNF7


Home / Products / Recombinant Proteins / Recombinant Human CUL5/RNF7

Recombinant Human CUL5/RNF7

CUL5 Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : CUL5-149H
Product Overview : Recombinant human CUL5 (amino acid residues 1-780), Rnf7 (amino acid residues 1-113) was expressed in Insect (Sf21) cells.
Description : Cullin-RING-Ligases (CRLs) are one largest class of ubiquitin E3 ligases and the enzymes of the NEDDylation pathway play a pivotal role in the activation of these, akin to ubiquitylation, the E1 activating enzyme (APP-BP1/UBA3 heterodimer) and the E2 conjugating enzymes (UBE2M or UBE2F) are involved in mammalian NEDDylation of the Cullin Ring Ligases (CRLs) (Meyer-Schaller et al., 2009; Huang et al., 2011; Morimoto et al., 2003).
Source : Insect cells
Species : Human
Form : 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity : E3 Ligase Assay: The activity of Cul5/Rnf7 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase His-DCNL1 and thioester loaded His-Ube2M-NEDD8. Incubation of Cul5/Rnf7 and thioester loaded His-Ube2M-NEDD8 in the presence or absence of His-DCNL1 at 4?C was compared at two time points T0 and T2 minutes. Increased Neddylation of the Cul5 subunit in the presence of His-DCNL1 was demonstrated.
Molecular Mass : Cul5=121kDa; Rnf7=13kDa
Storage : 12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration : 0.5 mg/ml
Gene Name : CUL5 cullin 5 [ Homo sapiens ]
Official Symbol : CUL5
Synonyms : CUL5; cullin 5; cullin-5; VACM 1; CUL-5; Vasopressin-activated calcium-mobilizing receptor-1; vasopressin-activated calcium-mobilizing receptor 1; Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1); VACM1; VACM-1;
Gene ID : 8065
mRNA Refseq : NM_003478
Protein Refseq : NP_003469
MIM : 601741
UniProt ID : Q93034
Chromosome Location : 11q22.3
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Disease, organism-specific biosystem; ECS complex, organism-specific biosystem;
Function : calcium channel activity; protein binding; receptor activity; ubiquitin protein ligase binding;

Online Inquiry

  • Note: There will be extra charge for optional service!
  • Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2007 – 2020 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy