Active Recombinant Human NAE1/UBA3
Cat.No. : | NAE1-168H |
Product Overview : | Recombinant human NAE1 (amino acid residues 3-536) and UBA3 (amino acid residues 22-463), was co-expressed in Insect sf21. |
- Specification
- Gene Information
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Description : | SAE1/SAE2 is a SUMO1, 2 and 3 E1 activating enzyme and functions as a heterodimer. Cloning of the human SAE1 and SAE2 genes was first described by Desterro et al. (1999). SAE1 and SAE2 share sequence similarity to the N-terminus and C-terminus of ubiquitin E1 activating enzymes respectively (Desterro et al. 1999). SAE2 harbours the E1-like active cysteine site while SUMO1 transfer to the E2 conjugating enzyme UBE2I requires both of the SAE subunits (Desterro et al. 1999). A crystal structure of the SAE1/SAE2 dimer together with the SUMO1 adenylate has been solved at 2.45 ?ngstr?m resolution (Olsen et al. 2010). |
Source : | Insect cells |
Species : | Human |
Form : | 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol |
Bio-activity : | E1 Thioester NEDD8 Loading Assay: The activity of APP-BP1/UBA3 was validated by loading NEDD8 onto the active cysteine of APP-BP1/UBA3. Incubation of the APP-BP1/UBA3 enzyme in the presence of NEDD8 and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the NEDD8 / APP-BP1/UBA3 thioester bond to the reducing agent DTT was confirmed. |
Molecular Mass : | APP-BP1 = 60.46 kDa UBA3 = 49.35 kDa |
Purity : | >98% by SDS-PAGE |
Storage : | 12 months at -70°C. Avoid multiple freeze/thaw cycles. |
Concentration : | 0.5 mg/ml |
Gene Name : | NAE1 NEDD8 activating enzyme E1 subunit 1 [ Homo sapiens ] |
Official Symbol : | NAE1 |
Synonyms : | NAE1; NEDD8 activating enzyme E1 subunit 1; amyloid beta precursor protein binding protein 1 , amyloid beta precursor protein binding protein 1, 59kDa , amyloid beta precursor protein binding protein 1, 59kD , APPBP1; NEDD8-activating enzyme E1 regulatory subunit; NEDD8 activating enzyme E1 subunit; ula 1; APP-BP1; protooncogene protein 1; proto-oncogene protein 1; amyloid protein-binding protein 1; NEDD8-activating enzyme E1 subunit; amyloid beta precursor protein-binding protein 1, 59kD; amyloid beta precursor protein binding protein 1, 59kDa; amyloid beta precursor protein-binding protein 1, 59 kDa; HPP1; ula-1; APPBP1; A-116A10.1; |
Gene ID : | 8883 |
mRNA Refseq : | NM_001018159 |
Protein Refseq : | NP_001018169 |
MIM : | 603385 |
UniProt ID : | Q13564 |
Chromosome Location : | 16q22 |
Pathway : | Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; |
Function : | contributes_to NEDD8 activating enzyme activity; catalytic activity; nucleotide binding; protein binding; protein heterodimerization activity; |
Products Types
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NAE1-417H | Recombinant Human NAE1 Protein, His-tagged | +Inquiry |
NAE1-3548R | Recombinant Rat NAE1 Protein, His (Fc)-Avi-tagged | +Inquiry |
NAE1-469C | Recombinant Cynomolgus Monkey NAE1 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
NAE1-3984HCL | Recombinant Human NAE1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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