"SAE1" Related Products


Recombinant Human SAE1/UBA2, GST/His-tagged

Cat.No.: SAE1-170H
Product Overview: Recombinant human SAE1 (amino acid residues 1-346), fused with N-terminal GST, Human UBA2 (amino acid residues 1-640), fused with N-terminal His, was expressed in E.coli.
Description: SAE1/SAE2 is a SUMO1, 2 and 3 E1 activating enzyme and functions as a heterodimer. Cloning of the human SAE1 and SAE2 genes was first described by Desterro et al. (1999). SAE1 and SAE2 share sequence similarity to the N-terminus and C-terminus of ubiquitin E1 activating enzymes respectively (Desterro et al. 1999). SAE2 harbours the E1-like active cysteine site while SUMO1 transfer to the E2 conjugating enzyme UBE2I requires both of the SAE subunits (Desterro et al. 1999).
Source: E. coli
Species: Human
Tag: GST/His
Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity: E1 Thioester SUMO Loading Assay: The activity of GST-SAE1/His-SAE2 was validated by loading SUMO1 onto the active cysteine of GST-SAE1/His-SAE2. Incubation of the GST-SAE1/His-SAE2 enzyme in the presence of SUMO1 and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the SUMO / GST-SAE1/His-SAE2 thioester bond to the reducing agent DTT was confirmed.
Molecular Mass: SAE1 = 64.8 kDa SAE2 = 73.4 kDa
Purity: >98% by SDS-PAGE
Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration: 0.5 mg/ml
Gene Name: SAE1 SUMO1 activating enzyme subunit 1 [ Homo sapiens ]
Official Symbol: SAE1
Synonyms: SAE1; SUMO1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; activator Of sumo 1; AOS1; FLJ3091; Sua1; activator of SUMO1; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; SUA1; UBLE1A; HSPC140;
Gene ID: 10055
mRNA Refseq: NM_001145713
Protein Refseq: NP_001139185
MIM: 613294
UniProt ID: Q9UBE0
Chromosome Location: 19q13.32
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem;
Function: ATP-dependent protein binding; contributes_to SUMO activating enzyme activity; enzyme activator activity; ligase activity; nucleotide binding; protein C-terminus binding; protein binding; protein heterodimerization activity; protein heterodimerization activity; ubiquitin activating enzyme activity;

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