"UCHL5" Related Products

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Recombinant Human UCHL5, GST-tagged

Cat.No.: UCHL5-181H
Product Overview: Recombinant human UCHL5 (amino acid residues 1-328), fused with N-terminal GST, was expressed in E.coli.
Description: The deubiquitylating activity of the proteasome has been attributed to the action of three deubiquitylases: UCHL5, USP14 and RPN11, which are all localised in the 19S regulatory particle. It has been reported that loss of both UCHL5 and USP14 leads to the accumulation of polyubiquitylated proteins and an inhibition of protein degradation without altering the structure or catalytic properties of the proteasome (D"Arcy et al., 2011).
Source: E. coli
Species: Human
Tag: GST
Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity: Deubiquitylase Enzyme Assay: The activity of GST-UCHL5 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-UCHL5 was compared confirming the deubiquitylating activity of GST-UCHL5.
Molecular Mass: ~64 kDa
Purity: >88% by SDS-PAGE
Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration: 0.5 mg/ml
Gene Name: UCHL5 ubiquitin carboxyl-terminal hydrolase L5 [ Homo sapiens ]
Official Symbol: UCHL5
Synonyms: UCHL5; ubiquitin carboxyl-terminal hydrolase L5; ubiquitin carboxyl-terminal hydrolase isozyme L5; CGI 70; INO80 complex subunit R; INO80R; UCH37; ubiquitin thioesterase L5; ubiquitin C-terminal hydrolase UCH37; ubiquitin carboxyl-terminal esterase L5; CGI-70; UCH-L5;
Gene ID: 51377
mRNA Refseq: NM_001199261
Protein Refseq: NP_001186190
MIM: 610667
UniProt ID: Q9Y5K5
Chromosome Location: 1q32
Function: cysteine-type peptidase activity; endopeptidase inhibitor activity; omega peptidase activity; peptidase activity; proteasome binding; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity;

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