Active Recombinant Human UCHL5, GST-tagged

• Cat.No.: UCHL5-181H
• Product Overview: Recombinant human UCHL5 (amino acid residues 1-328), fused with N-terminal GST, was expressed in E.coli.

Specification

• Description: The deubiquitylating activity of the proteasome has been attributed to the action of three deubiquitylases: UCHL5, USP14 and RPN11, which are all localised in the 19S regulatory particle. It has been reported that loss of both UCHL5 and USP14 leads to the accumulation of polyubiquitylated proteins and an inhibition of protein degradation without altering the structure or catalytic properties of the proteasome (D"Arcy et al., 2011).
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of GST-UCHL5 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-UCHL5 was compared confirming the deubiquitylating activity of GST-UCHL5.
• Molecular Mass: ~64 kDa
• Purity: >88% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: UCHL5 ubiquitin carboxyl-terminal hydrolase L5 [ Homo sapiens ]
• Official Symbol: UCHL5
• Synonyms: UCHL5; ubiquitin carboxyl-terminal hydrolase L5; ubiquitin carboxyl-terminal hydrolase isozyme L5; CGI 70; INO80 complex subunit R; INO80R; UCH37; ubiquitin thioesterase L5; ubiquitin C-terminal hydrolase UCH37; ubiquitin carboxyl-terminal esterase L5; CGI-70; UCH-L5
• Gene ID: 51377
• mRNA Refseq: NM_001199261
• Protein Refseq: NP_001186190
• MIM: 610667
• UniProt ID: Q9Y5K5
• Chromosome Location: 1q32
• Function: cysteine-type peptidase activity; endopeptidase inhibitor activity; omega peptidase activity; peptidase activity; proteasome binding; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity

Reviews

02/25/2023

The reproducibility is very good and I get consistent results every time, which makes me feel more confident in the results.

09/07/2020

The activity of UCHL5 is maintained for a long time, which ensures the stability of the experimental results.

03/25/2020

Excellent performance in Western blot, avoiding interference problems in experiments.

Q&As

How is the expression level of UCHL5 protein regulated?

The expression level of UCHL5 protein may be regulated by a variety of factors, including gene transcription, translation, post-transcriptional modification, etc. Studying these regulatory mechanisms can help to understand the expression patterns of UCHL5 protein in organisms and its effects on biological processes.

What is the expression pattern of UCHL5 protein?

UCHL5 protein may be highly expressed in specific tissues and cells and low in other tissues and cells. Its expression pattern can be influenced by a variety of factors, including gene regulation, transcription factors, and/or signal transduction pathways.

What are the effects of mutations or modifications in the UCHL5 protein on organisms?

Mutations or modifications of the UCHL5 protein may affect its function and thus affect the organism. The specific effects may vary depending on the type and location of the mutation or modification and may involve various biological processes, such as growth and development, immune response, etc.

What biological processes does UCHL5 protein play a role in?

This protein may be involved in a variety of biological processes, including protein quality control, cell cycle regulation, DNA repair, and signal transduction. These processes often require the involvement of UPS to achieve protein degradation and regulation.

How does UCHL5 protein interact with other proteins?

UCHL5 proteins may interact with other proteins to fulfill their functions. These interactions can involve various types of protein-protein interactions like electrostatic interactions, hydrogen bonding, etc. Understanding these interactions can help unravel the mechanism of action of the UCHL5 protein in cells.

What is the function of UCHL5 protein?

The main function of UCHL5 protein may be as a component of the ubiquitin-proteasome system (UPS), which is involved in the regulation of protein quality control, cell cycle regulation, DNA repair, and signal transduction. It may perform these functions by hydrolyzing specific substrates.