Recombinant Full Length Human UCHL5 Protein, C-Flag-tagged

• Cat.No.: UCHL5-2070HFL
• Product Overview: Recombinant Full Length Human UCHL5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables endopeptidase inhibitor activity; proteasome binding activity; and thiol-dependent deubiquitinase. Involved in negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of smoothened signaling pathway; and protein deubiquitination. Located in cytosol; nucleolus; and nucleoplasm. Colocalizes with Ino80 complex.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 37.4 kDa
• AA Sequence: MTGNAGEWCLMESDPGVFTELIKGF GCRGAQVEEIWSLEPENFEKLKPVH GLIFLFKWQPGEEPAGSVVQ DSRLDTIFFAKQVINNACATQAIVS VLLNCTHQDVHLGETLSEFKEFSQS FDAAMKGLALSNSDVIRQVH NSFARQQMFEFDTKTSAKEEDAFHF VSYVPVNGRLYELDGLREGPIDLGA CNQDDWISAVRPVIEKRIQK YSEGEIRFNLMAIVSDRKMIYEQKI AELQRQLAEEEPMDTDQGNSMLSAI QSEVAKNQMLIEEEVQKLKR YKIENIRRKHNYLPFIMELLKTLAE HQQLIPLVEKAKEKQNAKKAQETK TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease

Gene Information

• Gene Name: UCHL5 ubiquitin C-terminal hydrolase L5 [ Homo sapiens (human) ]
• Official Symbol: UCHL5
• Synonyms: Enables endopeptidase inhibitor activity; proteasome binding activity; and thiol-dependent deubiquitinase. Involved in negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of smoothened signaling pathway; and protein deubiquitination. Located in cytosol; nucleolus; and nucleoplasm. Colocalizes with Ino80 complex.
• Gene ID: 51377
• mRNA Refseq: NM_015984.5
• Protein Refseq: NP_057068.1
• MIM: 610667
• UniProt ID: Q9Y5K5