Active Recombinant Human TET1, Flag-tagged

• Cat.No.: TET1-997H
• Product Overview: Recombinant Human TET1(1418 a.a. - 2136 a.a.), fused with FLAG-tag at N-terminal, was expressed in Baculovirus infected sf9 cell expression system.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Flag
• Protein Length: 1418-2136 a.a.
• Description: Ten-eleven translocation methylcytosine dioxygenase 1 (TET1) is a member of the TET family of enzymes that in humans is encoded by the TET1 gene. TET1 catalyzes the conversion of the modified DNA base 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC). TET1 produces 5-hmC by oxidation of 5-mC in an iron and alpha-ketoglutarate dependent manner. The conversion of 5-mC to 5-hmC has been proposed as the initial step of active DNA demethylation in mammals.
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol in the elution buffer.
• Molecular Mass: 80 kDa
• Purity: >80% purity
• Applications: Active DNA demethylase can be used for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing. Best when used within six months from the date of receipt.

Gene Information

• Gene Name: TET1 tet methylcytosine dioxygenase 1 [ Homo sapiens (human) ]
• Official Symbol: TET1
• Synonyms: TET1; tet methylcytosine dioxygenase 1; LCX; CXXC6; bA119F7.1; methylcytosine dioxygenase TET1; CXXC finger 6; tet oncogene 1; CXXC zinc finger 6; TET1 splice variant VP_DE4; ten-eleven translocation-1; TET1 splice variant VP_DE456; CXXC-type zinc finger protein 6; ten-eleven translocation 1 gene protein; leukemia-associated protein with a CXXC domain; NP_085128.2; EC 1.14.11.n2
• Gene ID: 80312
• mRNA Refseq: NM_030625
• Protein Refseq: NP_085128
• MIM: 607790
• UniProt ID: Q8NFU7
• Chromosome Location: 10q21
• Pathway: Epigenetic regulation of gene expression; Gene Expression; TET1,2,3 and TDG demethylate DNA
• Function: iron ion binding; methylcytosine dioxygenase activity; structure-specific DNA binding