Description : |
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. |
Source : |
E.coli |
Species : |
Pig |
Form : |
Lyophilized from a 0.2μm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT. |
Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg in the presence of 75 pg/ml rPoIL-1α. |
Molecular Mass : |
Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
Protein length : |
152 |
AA Sequence : |
HPLGKRPCRMQAFRIWDVNQKTFYL RNNQLVAGYLQGPNTKLEEKIDVVP VEPHFVFLGIHGGKLCLSCVKSGDE MKLQLDAVNITDLRKNSEQDKRFTF IRSDSGPTTSFESAACPGWFLCTAL EADQPVGLTNTPKAAVKVTKFYFQQ DQ |
Endotoxin : |
Less than 0.1 EU/µg of rPoIL-1RA as determined by LAL method. |
Purity : |
>97% by SDS-PAGE and HPLC analysis. |
Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |