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Recombinant Protease

Cat.No. : Protease-92
Product Overview : Recombinant Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well. The Recombinant Protease is purified by proprietary chromatographic techniques.
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Cat. No. : Protease-92
Source : Escherichia Coli.
Physical Appearance : Sterile filtered colorless solution.
Cleavage Buffer : 50 mM Tris-HCl, pH-7.0 (at 25°C), 150 mM NaCl, 1 mM EDTA, 1mM dithiothreitol. Chill to 5°C prior to use.
Cleavage Conditions : For Cleavage of a Fusion Protein: During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease.
Unit Defenition : One unit will cleave ≥90% of 100 µg of a test GST-fusion protein in Cleavage Buffer (50mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 at 25°°C) at 5°C for 16 hours.
Stability : Recombinant Protease although stable at 14°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze thaw cycles.
Synonyms : Protease; Peptide Hydrolases; EC 3.4-; Glutamic acid proteases.
Synonyms : Protease; Peptide Hydrolases; EC 3.4-; Glutamic acid proteases.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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