||Recombinant human Survivin fused to CaM-tag at N-terminus was expressed in E.coli and purified by conventional chromatography techniques, 33 kDa (294 aa).
||Survivin is a human gene that is part of the inhibitor of apoptosis family (IAP). The Survivin protein functions to inhibit caspase activation therefore leading to negative regulation of apoptosis or programmed cell death.
|Sequences of amino acids:
||MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SHMGAPTLPP AWQPFLKDHR ISTFKNWPFL EGCACTPERM AEAGFIHCPT ENEPDLAQCF FCFKELEGWE PDDDPIEEHK KHSSGCAFLS VKKQFEELTL GEFLKLDRER AKNKIAKETN NKKKEFEETA KKVRRAIEQL AAMD
||Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl
||> 90% by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.