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Recombinant Human DnaJ (Hsp40) Homolog, Subfamily B, Member 1, His-tagged;

Cat. No.: DNAJB1-323H
Product Overview: Recombinant human HSP40, his-tagged, is overexpressed in E.coli and purified by using the conventional column chromatography techniques (360 aa,40.2 kDa).
Description: Human HSP40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. HSP40 protein contains the J-domain which controls interaction with the ATPase domain of DnaK.
Source: E.coli.
Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM DTT, 0.2 mM PMSF, 10% glycerol.
Molecular Weight: 40.2 kDa (360 aa).
Purity: > 95% by SDS – PAGE.
Concentration: 1 mg/ml (determined by Bradford assay).
Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
Sequence: MGSSHHHHHH SSGLVPRGSH MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: DNAJB1
Synonyms: DNAJB1; DnaJ (Hsp40) homolog, subfamily B, member 1; Hdj1; Sis1; HSPF1; Hsp40; DNAJ1; HDJ-1; HDJ1; DnaJ protein homolog 1; Heat shock protein 40; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; DnaJ (Hsp40) homolog, subfamily B, member 1; Hsp40 (heat shock protein 40 kD).
Gene ID: 3337
mRNA Refseq: NM_006145.1
Protein Refseq: NP_006136.1
MIM: 604572
UniProt ID: P25685
Chromosome Location: 19p13.2
Function: heat shock protein binding; unfolded protein binding.

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