Recombinant Human DPP4 Protein, His-tagged

• Cat.No.: DPP4-116H
• Product Overview: Recombinant human DPP4 protein(39-766aa) with His tag was expressed in insect cell.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 39-766 a.a.
• Description: The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.
• Form: Liquid
• Molecular Mass: 85.4kDa
• AA Sequence: ADPSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLPHHHHHH
• Endotoxin: < 1.0 EU per 1 μg of protein (determined by LAL method)
• Purity: > 95% by SDS - PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Absorbance at 280 nm)
• Storage Buffer: Liquid in 20mM Tris-HCl pH 8.0, 100mM NaCl, 1mM EDTA, 10% glycerol

Gene Information

• Gene Name: DPP4 dipeptidyl peptidase 4 [ Homo sapiens (human) ]
• Official Symbol: DPP4
• Synonyms: CD26; ADABP; ADCP2; DPPIV; TP103; dipeptidyl peptidase 4; ADCP-2; DPP IV; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); EC 3.4.14.5
• Gene ID: 1803
• mRNA Refseq: NM_001935
• Protein Refseq: NP_001926
• MIM: 102720
• UniProt ID: P27487

Citation

Peptide Array on Cellulose Support—A Screening Tool to Identify Peptides with Dipeptidyl-Peptidase IV Inhibitory Activity within the Sequence of α-Lactalbumin

Journal: International Journal of Molecular Sciences    PubMed ID: 25402645    Data: 2014/11/13

Authors: Isabelle M. E. Lacroix, Eunice C. Y. Li-Chan, Brijesh Tiwari

Article Snippet:Recombinant human dipeptidyl-peptidase IV (DPP-IV, EC 3.4.14.5, expressed in HEK293 human cells, >95% purity, activity >5500 pmoles Gly–Pro–7-amido-4-methylcoumarin/min/μg) was purchased from Creative BioMart (Shirley, NY, USA) while polyclonal goat DPP-IV antibody and polyclonal donkey anti-goat secondary antibody conjugated to horseradish peroxidase were obtained from R&D Systems (Minneapolis, MN, USA).. Pierce? ECL western blotting substrate and protein-free (TBS) blocking buffer were from Thermo Scientific (Rockford, IL, USA).Pierce? ECL western blotting substrate and protein-free (TBS) blocking buffer were from Thermo Scientific (Rockford, IL, USA).

Schematic representation of peptide array synthesis, binding and inhibition experiments. Binding of α-lactalbumin-derived decamers to the dipeptidyl-peptidase IV (DPP-IV) enzyme was investigated directly on the cellulose membrane, whereas the inhibition of DPP-IV was measured using a colorimetric assay with the cleaved peptides.

Binding of DPP-IV to deca-peptides derived from α-lactalbumin (A1–K4, ) and from other proteins (K5–K11, and ). The binding is inversely correlated to the light signal, the darkest spots representing peptides on which more enzyme was bound.

Correlation between binding of DPP-IV to deca-peptides on the array (indicated by luminescence intensity) and inhibition of the enzyme activity by the peptides.