"DPP4" Related Products

Recombinant Human Dipeptidyl peptidase-4, His-tagged

Cat. No.: DPP4-116H
Product Overview: Recombinant human DPP4 protein was expressed with c-terminal His-tag in high-5 cells using baculovirus expression system and purified by using conventional chromatography techniques, 86.4kDa (746aa).
Description: DPP4, dipeptidyl peptidase-4 is a complex enzyme expressed on the surface of most cell types and is a serine exopeptidase that cleaves x-proline dipeptides from the N-terminus of polypeptides. DPP4 protein is associated with intracellular signal transduction, apoptosis and plays an important role in tumor biology. There are at least 63 substrates which can bind specifically to DPP4 enzyme including growth factors, chemokines, neuro peptides. Furthermore, DPP4 plays a major role in glucose metabolism by cleaving incretins such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1).
Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1 mM EDTA, 10% glycerol
Purity: > 95% by SDS - PAGE
Concentration: 0.5 mg/ml (determined by Bradford assay)
Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method)
Unit Definition: One unit will hydrolyze 1 mmole of p-nitroaniline per minute at pH8.0 at 37℃ using 1mM of Gly-Pro p-nitroanilde as a substrate.One unit will hydolyze
Assay procedure and results: - Reaction buffer: 20mM Tris pH8.0, 0.1M NaCl, 1mM EDTA - Total reaction volume: 100 ul - Reaction temperature: 37℃ 1. Add the reaction buffer to each well 2. Add the 10 ul of 10mM substrate (Gly-Pro p-nitroanilide) to each well 3. Add the enzyme (DPP-4) diluent to each well 4. Incubate the 96 well plate at 37℃. 5. Read the optical density at 405 nm.
Biological activity: >20 Unit/mg
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: DPP4 dipeptidyl-peptidase 4 [ Homo sapiens ]
Synonyms: ADABP; ADCP2; CD26; DPPIV; EC 3.4.14.5; TP103; Adenosine deaminase complexing protein 2; CD26 antigen; DPP IV; Dipeptidyl peptidase IV; T-cell activation antigen CD26; dipeptidyl-peptidase 4; dipeptidylpeptidase 4; dipeptidylpeptidase IV; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4
Gene ID: 1803
mRNA Refseq: NM_001935
Protein Refseq: NP_001926
MIM: 102720
UniProt ID: P27487
Chromosome Location: 2q24.3
Function: aminopeptidase activity; dipeptidyl-peptidase activity; protein binding; homodimerization activity; serine-type endopeptidase activity

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